安徽医科大学学报2024，Vol.59Issue(7)：1116-1122,7.DOI:10.19405/j.cnki.issn1000-1492.2024.07.002
基于16sRNA测序分析亚油酸对小鼠肠道菌群的影响
The effects of linoleic acid on intestinal flora in mice were analyzed based on 16sRNA sequencing
摘要
Abstract
Objective To investigate the effects of linoleic acid(LA)on the diversity and structure of intestinal flora in mice.Methods Twelve SPF grade male C57BL/6J mice at 7 weeks were randomly divided into control group(CTRL group)and linoleic acid group(LA group).One day before the linoleic acid diet was supplemented,the normal food was removed from the LA group and the mice in the LA group were fasted for one night,so that the LA diet was more acceptable to the mice in the LA group,and LA was given on the day of the experiment recording,and the feed was updated at any time to ensure that the mice could eat freely until the end of modeling.After 12 weeks of modeling,mouse feces were collected,and mixed samples were collected for every two mice feces,and then 16sRNA high-throughput sequencing was performed to analyze intestinal flora structure,Alpha and Beta diversity.Results 16sRNA high-throughput sequencing showed that LA intervention damaged the richness and diversity of intestinal flora.The results of principal component analysis showed that the composition of flora in CTRL group was different from that in LA group.At gate level,the relative abundance of Actinobacteria in LA group increased(P＜0.01).At the genus level,the relative abundance of L.Duchennei in the LA group decreased(P＜0.05),but the relative abundance of Bifidobacterium,Faecalibaculum and Erysipelotrichaceae in the LA group increased(all P＜0.01).Conclusion LA intervention could reduce the richness and diversity of intestinal flora in mice,and adjust the structure of intestinal flora.There were significant differences between beneficial bacteria and pathogenic bacte-ria in intestinal flora after LA intervention,which provided certain basis for the treatment of bioactive compounds of linoleic acid and the therapeutic adjustment of intestinal microorganisms as targets.关键词
高通量测序/亚油酸/肠道菌群/代谢/多样性Key words
high-throughput sequencing/linoleic acid/gut flora/metabolism/diversity分类
医药卫生引用本文复制引用
李宗恒,张雪芳,陈延华,尚靖,骆莺,殷佩浩..基于16sRNA测序分析亚油酸对小鼠肠道菌群的影响[J].安徽医科大学学报,2024,59(7):1116-1122,7.基金项目
国家自然科学基金(编号:81873137) （编号:81873137）
