安徽医科大学学报2024，Vol.59Issue(7)：1128-1133,6.DOI:10.19405/j.cnki.issn1000-1492.2024.07.004
大麻二酚抑制创伤性脑损伤大鼠睾丸凋亡相关蛋白的表达
Cannabidiol inhibits the express of apoptosis-related proteins in the testis of rats with traumatic brain injury
摘要
Abstract
Objective To investigate testicular damage in traumatic brain injury(TBI)rats and to analyze the in-terventional effects of cannabidiol(CBD)on TBI-induced testicular damage.Methods 18 Sprague Dawley(SD)rats were randomly divided into three groups:the sham operation group(Sham group),model group(TBI group)and treatment group(TBI+CBD group).HE staining was used to observe the testicular histopathological changes in the rat testis.ELISA was used to detect testosterone level in rat serum.TUNEL staining was utilized to observe apoptosis,while immunofluorescence staining,Western blot and RT-qPCR were employed to evaluate Bax,Bcl-2,Cleaved Caspase-3 and TNF-α protein and mRNA expression.Results HE staining showed pathological changes in the testes of TBI rats compared with those in the Sham group.ELISA assay showed a decrease in testosterone levels in the TBI group compared to the Sham group,but there was no significant difference(P＞0.05).Immunofluores-cence results showed that the intensity of Bax fluorescence expression increased in the TBI group compared with the Sham group(P＜0.01),whereas the intensity of Bax fluorescence decreased and the intensity of Bcl-2 fluorescence increased in the rats after the CBD intervention(P＜0.01).Western Blot results showed that CBD treatment in rats decreased the protein levels of testicular apoptosis-related proteins(Bax and Cleaved Caspase-3,all P＜0.05)and inflammation-related proteins(TNF-α,P＜0.01),and increased the protein level of the anti-apoptotic protein,Bcl-2(P＜0.05).The trend of RT-qPCR results was similar,with mRNA expression of Bax(P＜0.05)and TNF-α(P＜0.01)decreased and mRNA expression of Bcl-2 increased after CBD intervention compared with the TBI group(P＜0.05).Conclusion TBI induces testis injury,and CBD treatment effectively repairs apoptosis and in-flammatory in testicular tissue of TBI rats.关键词
大麻二酚/创伤性脑损伤/凋亡/炎症/大鼠/睾丸Key words
cannabidiol/traumatic brain injury/apoptosis/inflammatory/rats/testis分类
医药卫生引用本文复制引用
李佳丽,凌腾晗,曹艳,尹爱平,李恒希,杨莉,吴海鹰,李坪..大麻二酚抑制创伤性脑损伤大鼠睾丸凋亡相关蛋白的表达[J].安徽医科大学学报,2024,59(7):1128-1133,6.基金项目
国家自然科学基金(编号:82060241、82260387) （编号:82060241、82260387）
云南省科技厅计划项目(编号:202101AY070001-002) （编号:202101AY070001-002）
云南省医疗机构科研项目(编号:202403AC100010) （编号:202403AC100010）
昆明医科大学研究生创新基金(编号:2023S133) （编号:2023S133）
