安徽医科大学学报2024，Vol.59Issue(7)：1175-1180,6.DOI:10.19405/j.cnki.issn1000-1492.2024.07.010
基于Cre/Loxp系统的Csf1r-CreERT2 R26REYFP报告基因小鼠的构建及效率检测
Construction and efficiency detection of Csf1r-CreERT2 R26REYFP reporter gene mouse based on Cre/Loxp system
摘要
Abstract
Objective To construct Csf1r-CreERT2 R26REYFP reporter gene mice and assess the efficacy of Csf1r-CreERT2-mediated enhancement of CSF1R in CD45+cells labeled with yellow fluorescein protein EYFP.Methods Csf1r-CreERT2 mice were crossbred with R26REYFP homozygous mice,and Csf1r-CreERT2R26REYFP mice were identified through PCR and Western Blot analyses.Flow cytometry was employed to evaluate CSF1R tag-efficiency in CD45+cells across different mouse tissues following tamoxifen induction.Results Csf1r-CreERT2 R26REYFP reporter gene mice were acquired.In addition,it was found that Csf1r-CreERT2-mediated EYFP could effectively mark CSF1R in various tissues of mice and CD45+cells in different locations.Compared to the R26REYF P group,the highest labeling efficiency was observed in the brain tissue(P＜0.001),the lowest in the thymus tissue(P＜0.05),and no sig-nificant difference was observed in the spleen tissue.Conclusion Adult Csf1r-CreERT2 mice and R26REYFP mice are effective ways to obtain Csf1r-CreERT2 R26REYFP induced conditional fluorescence mice.Csf1r-CreERT2 can mediate EYFP to effectively trace CSF1R in CD45+cells in different parts of mice.关键词
Csf1r-CreERn/R26REYFP/Cre/LoxP 系统/CD45/流式细胞术Key words
Csf1r-CreERT2/R26REYFP/Cre/LoxP system/CD45/flow cytometry分类
医药卫生引用本文复制引用
朱向玲,吴旭铭,王卉卉,周园园,王安琪,张慧茹,刘崇,涂佳杰..基于Cre/Loxp系统的Csf1r-CreERT2 R26REYFP报告基因小鼠的构建及效率检测[J].安徽医科大学学报,2024,59(7):1175-1180,6.基金项目
安徽省教育厅高校科学研究项目(编号:2022AH020052) （编号:2022AH020052）
