安徽医科大学学报2024，Vol.59Issue(7)：1195-1200,6.DOI:10.19405/j.cnki.issn1000-1492.2024.07.013
内皮祖细胞外泌体中miRNA-222-3p对糖尿病小鼠皮肤伤口愈合的影响
Effect of miR-222-3p in endothelial progenitor cell-derived exosomes on skin wound healing in diabetic mice
摘要
Abstract
Objective To explore the effect of miRNA-222-3p in endothelial progenitor cell-derived exosomes(EPCs-Exo)on skin wound healing in diabetic mice.Methods Endothelial progenitor cell(EPCs)derived from C57BL/6 mouse bone marrow were identified by fluorescence staining.Subsequently,EPCs-Exo isolated from the media of EPCs were identified,and high-throughput sequencing of EPCs-Exo miRNA was completed.The skin injury model of diabetic mice was established.According to different groups,the wounds were treated with externally ap-plied phosphate buffer solution(PBS)and EPCs-Exo;Subcutaneous injection of PBS,agomiR-222-3p,and an-tagomiR-222-3p at the edge of the wound was performed continuously for 14 days,and the wound healing rate was observed.Meanwhile,immunofluorescence methods were used to investigate the changes in the expression levels of ROS,CD31,and VEGF in the wound margin tissue before and after treatment.Results EPCs-Exo significantly ac-celerated skin wound healing in diabetic mice,reduced the level of ROS,and increased the expression level of VEGF and CD31 in the wound margin tissue(P＜0.05).High-throughput sequencing showed that miRNA-222-3p was highly expressed in EPCs-Exo.Local subcutaneous injection of miRNA-222-3p into wound margin tissue signifi-cantly promoted the skin wound healing of diabetic mice,reduced the level of ROS,and increased the expression level of VEGF and CD31 in the wound margin tissue(P＜0.05).Conclusion MiRNA-222-3p promotes wound healing in diabetic mice and plays an important role in EPCs-Exo promoting wound healing.关键词
内皮祖细胞/外泌体/糖尿病足/miRNA-222-3p/创面愈合Key words
endothelial progenitor cells/exosome/diabetic foot/miRNA-221-3 p/wound healing分类
医药卫生引用本文复制引用
王永琪,季华,唐颖,巫婷婷,揭茹艳,陈明卫..内皮祖细胞外泌体中miRNA-222-3p对糖尿病小鼠皮肤伤口愈合的影响[J].安徽医科大学学报,2024,59(7):1195-1200,6.基金项目
安徽省自然科学基金(编号:2108085MH269) （编号:2108085MH269）
