安徽医科大学学报2024，Vol.59Issue(7)：1201-1205,1212,6.DOI:10.19405/j.cnki.issn1000-1492.2024.07.014
虎杖苷通过调节JAK2/STAT3信号通路改善血管内皮细胞损伤的研究
Polydatin protects vascular endothelial cells by modifying the JAK2/STAT3 signaling pathway
摘要
Abstract
Objective To study the protective effect of polydatin on lipopolysaccharide-induced injury of human umbilical vein vascular endothelial cells(HUVECs)through the protein Janus kinase 2(JAK2)/signal transducers and activators of transcription 3(STAT3)signaling pathway.Methods HUVECs were cultured in vitro,and 500 ng/ml LPS induced their injury and set as a model group;based on the model group,endothelial cells were inter-vened with different concentrations(10,20,and 40 μmol/L)of polydatin for 24 h,and set as polydatin low concentration group,polydatin medium concentration group,and polydatin high concentration group,respectively;a control group was set as another group.CCK-8,monocyte-endothelial cell adhesion,scratch and Transwell assays were used to detect cell viability,adhesion,migration and invasive ability;ELISA was used to detect interleukin-6(IL-6)and tumor necrosis factor-alpha(TNF-α)levels in the cell supernatant;Western blot was used to detect the expression of proteins related to the JAK2/STAT3 signaling pathway levels of JAK2/STAT3 signaling pathway relat-ed proteins.Results Compared with the control group,the model group showed decreased cell survival(P＜0.01),increased cell adhesion,migration and invasion(P＜0.001,P＜0.05,P＜0.01),increased levels of IL-6 and TNF-α in the cell supernatant(P＜0.001),and increased levels of phosphorylation of JAK2 and STAT3 pro-teins in the cells(P＜0.05).Compared with the model group,LPS damage to cells was attenuated after polydatin intervention,cell survival was increased in polydatin low-,medium-and high-concentration groups(P＜0.05),cell adhesion,migration,and invasion decreased(P＜0.05,P＜0.05,P＜0.001),IL-6 and TNF-α levels in cell supernatants decreased(P＜0.05),and the levels of cellular JAK2 and STAT3 protein phosphorylation lev-els decreased(P＜0.05).Conclusion Polydatin seems to reduce the inflammatory injury of human umbilical vein endothelial cells induced by LPS,reducing the secretion of inflammatory factors,and inhibiting the ability of cell ad-hesion,migration and invasion,which may be related to the down-regulation of JAK2/STAT3 signal pathway by polydatin.关键词
动脉粥样硬化/人脐静脉内皮细胞/虎杖苷/JAK2/STAT3信号通路Key words
atherosclerosis/human umbilical vein endothelial cells/polydatin/JAK2/STAT3 signaling pathway分类
临床医学引用本文复制引用
孔稳稳,韦惠珍,徐媛颖,沙雯君,鲁郡,雷涛..虎杖苷通过调节JAK2/STAT3信号通路改善血管内皮细胞损伤的研究[J].安徽医科大学学报,2024,59(7):1201-1205,1212,6.基金项目
国家自然科学基金面上项目(编号:81774083) （编号:81774083）
上海市普陀区卫生健康系统临床特色专科建设项目(编号:2020tszk01) （编号:2020tszk01）
上海市医学重点专科建设项目(编号:ZK2019B16) （编号:ZK2019B16）
上海市普陀区卫生健康系统科技创新项目(编号:ptkwws202003) （编号:ptkwws202003）
上海市卫生健康委员会课题(编号:202240309) （编号:202240309）
