慢性阻塞性肺疾病患者外周血中冷诱导RNA结合蛋白和髓样细胞触发受体-1的表达水平及其临床意义OA北大核心CSTPCD
The expression levels and clinical significance of cold induced RNA binding protein and myeloid cell trigger receptor-1 in peripheral blood of patients with chronic obstructive pulmonary disease
目的 探讨慢性阻塞性肺疾病(COPD)患者外周血中冷诱导RNA结合蛋白(CIRBP)和髓样细胞触发受体-1(TREM-1)的表达水平及其临床意义.方法 选取2022年10月—2023年6月40例住院的COPD患者为急性加重期组,其经过治疗进入稳定期后纳入稳定期组,同期40例健康体检者为对照组.收集各组一般资料,采集外周血,通过酶联免疫吸附法测定血浆中CIRBP、TREM-1水平,采用实时荧光定量聚合酶链式反应(qRT-PCR)法测定CIRBP mRNA和TREM-1 mRNA的相对表达量,并检测COPD患者和健康体检者的肺功能.结果 COPD急性加重期外周血中的CIR-BP、TREM-1表达水平均高于稳定期组,差异有统计学意义(均P＜0.001);急性加重期和稳定期外周血中的CIRBP、TREM-1表达水平均高于对照组,差异有统计学意义(均P＜0.001);在急性加重期和稳定期,CIRBP水平均与TREM-1水平呈正相关;急性加重期CIRBP、TREM-1水平与白细胞计数、中性粒细胞百分比、中性粒细胞/淋巴细胞比率呈正相关;稳定期CIRBP、TREM-1水平与第1秒用力呼气容积占预计值百分比、第1秒用力呼气容积/用力肺活量比值呈负相关,与白细胞计数、中性粒细胞百分比、中性粒细胞/淋巴细胞比率呈正相关.结论 CIRBP和TREM-1的表达水平在COPD患者外周血中升高,CIRBP的表达与TREM-1表达具有相关性,并且与肺功能、白细胞计数、中性粒细胞百分比、中性粒细胞与淋巴细胞比率、单核细胞与高密度脂蛋白比率等临床指标相关,提示CIRBP和TREM-1可能共同参与COPD的发生发展.
Objective To investigate the expression levels and clinical significance of cold-inducible RNA-binding protein(CIRBP)and triggering receptor expressed on myeloid cells-1(TREM-1)in peripheral blood of patients with chronic obstructive pulmonary disease(COPD).Methods Forty hospitalized COPD patients from October 2022 to June 2023 were selected as the acute exacerbation group.After treatment,they entered the stable phase and were included in the stable phase group.During the same period,40 healthy individuals underwent physical exami-nations as the control group.General information from each group was collected,peripheral blood was collected,and the levels of CIRBP and TREM-1 in plasma were measured using enzyme-linked immunosorbent assay(ELISA).The relative expression levels of CIRBP mRNA and TREM-1 mRNA were measured using real-time fluorescence quantitative polymerase chain reaction(qRT-PCR),and lung function was measured in COPD patients and healthy individuals.Results The expression levels of CIRBP and TREM-1 in peripheral blood during the acute exacerba-tion of COPD were higher than those in the stable phase group,and the differences were statistically significant(all P＜0.001);The expression levels of CIRBP and TREM-1 in peripheral blood during acute exacerbation and stable phases were higher than those in the control group,and the differences were statistically significant(all P＜0.001);In both acute exacerbation and stable phases,CIRBP levels were positively correlated with TREM-1 levels;The levels of CIRBP and TREM-1 during acute exacerbation were positively correlated with white blood cell count,neutrophil percentage,neutrophil to lymphocyte ratio;The stable CIRBP and TREM-1 levels were negatively correla-ted with the percentage of forced expiratory volume at 1 second to the expected value,and the ratio of forced expira-tory volume to forced vital capacity at 1 second.They were positively correlated with white blood cell count,neutro-phil percentage,and neutrophil/lymphocyte ratio.Conclusion The expression levels of CIRBP and TREM-1 are el-evated in the peripheral blood of COPD patients.The expression of CIRBP is correlated with TREM-1 expression,and is associated with clinical indicators such as lung function,white blood cell count,neutrophil percentage,neutro-phil to lymphocyte ratio,monocyte to high-density lipoprotein ratio,etc.,suggesting that CIRBP and TREM-1 may jointly participate in the occurrence and development of COPD.
张政;陈雪儿;卢献灵
石河子大学医学院内科学,石河子 832000石河子大学第一附属医院呼吸与危重症医学科,石河子 832000
临床医学
慢性阻塞性肺疾病冷诱导RNA结合蛋白髓样细胞触发受体-1
chronic obstructive pulmonary diseasecold-inducible RNA binding proteintriggering receptor ex-pressed on myeloid cells-1
《安徽医科大学学报》 2024 (007)
1275-1280 / 6
2022年度兵团指导性科技计划项目(编号:2022ZD039)
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