中医康复2024，Vol.1Issue(2)：1-7,7.DOI:10.19787/j.issn.2097-3128.2024.02.001
Collagen Ⅴ在推拿促进长期大负荷运动大鼠肌肉干细胞自我更新中的作用研究
The Role of Collagen Ⅴ in Tuina Promoting Self-Renewal of Muscle Satellite Cells Undergoing Long-Term Large Load Exercise in Rats
摘要
Abstract
Objective:To observe the effects of Tuina on the repair of skeletal muscle injury,grasping force of hind limbs and expression of CollagenⅤ(COLⅤ)after long-term large load exercise in rats,and to explore the mechanism of Tuina promoting the self-renewal of muscle satellite cells(MSC).Methods:A total of 32 male Sprague-Dawley rats were randomly divided into groups C,E,ET,and ES.Group C did not do any treatment,while the other rats did large load treadmill exercise for 4 weeks to prepare gastrocnemius injury model.Group E received no intervention.Group ET received Tuina 6h after each exercise,while group ES only received stroke.Body weight and grasping force of hind limbs of the rats were measured at the beginning and 4 weeks later.after 24h of the last exercise and Tuina,samples of gastrocnemius were obtained from both sides of all the rats,and observed the ultrastructure of skeletal muscle fibers by transmission electron microscopy,detected the expression of Collagen Ⅴ with immunohis-tochemical staining,the expression of mRNA Col5al and Co15a3 with RT-PCR,and the expression of Pax7,MyoD and Myf5 protein with Western blotting,observed co-localization by labeled Pax7 and Collagen Ⅴ with Double immunofluorescence staining.Results:Compared with group C,the increased grasping force of hind limbs in group E,ES and ET significantly different(P＜0.01),the changes such as myofibril fracture were observed in the ultrastructure of group E and ES,and the expression of Collagen Ⅴ,Pax7,MyoD,Myf5 protein,mRNA Col5al,Col5a3 and the expressions of co-location of Pax7 and Collagen Ⅴ in group E and ES decreased significantly(P＜0.05).Compared with group E,the increased grasping force of hind limbs in group ET significantly different(P＜0.05),the expression of Collagen Ⅴ,Pax7,MyoD,Myf5 protein,mRNA Col5al,Col5a3 and the ex-pressions of co-location of Pax7 and Collagen Ⅴ in group ET increased significantly(P＜0.05),and the ultrastructural damage recovered well.Conclu-sion:Tuina can promote the self-renewal of skeletal muscle satellite cells in rats by up-regulating expression of Collagen Ⅴ,to accelerate the repair of ultrastructural damage induced by long-term large load exercise and enhancing muscle strength.关键词
长期大负荷运动/推拿/肌肉干细胞/自我更新/V型胶原蛋白Key words
long-term large load exercise/Tuina/muscle satellite cells/self-renewal/Collagen Ⅴ分类
医药卫生引用本文复制引用
张瑞驰,靳松林,李伦宇,阿衣留布,丁海丽..Collagen Ⅴ在推拿促进长期大负荷运动大鼠肌肉干细胞自我更新中的作用研究[J].中医康复,2024,1(2):1-7,7.基金项目
国家自然科学基金项目,编号:81904318 （）
