北京大学学报（自然科学版）2024，Vol.60Issue(4)：701-712,12.DOI:10.13209/j.0479-8023.2023.061
给水系统病毒群落及其潜在宿主和功能基因的分布特征
Distribution Characteristics of Viral Community and Their Potential Hosts and Functional Genes in a Water Supply System
摘要
Abstract
Based on metagenomic shotgun sequencing,the viral community structure,potential hosts,and specific functional genes in key monitoring sites of a typical drinking water supply system were explored.The result revealed the presence of massive unclassified viral species,with the dominance of double-stranded DNA viruses occupying 94％-100％ of viral abundance.The phages Siphoviridae and Myoviridae were identified as the major known taxa.Conventional and advanced treatment processes reduced viral richness,diversity,and replication potentials,while the richness and diversity slightly increased after passing water supply pipe network.Both treatment processes restrained the roles of viruses in mediating the exchange of auxiliary metabolic genes (AMGs).Viral genomes in source water possessed various AMGs involved in energy metabolism and biosynthesis of carbohydrates,amino acids,and cofactors,which could contribute to bacterial host metabolization.However,after conventional treatment,they mainly encoded penicillin-binding proteins.Notably,in the water sample with a long-term exposure to hospital clinic settings,the abundance of viruses (35％) carrying antibiotic resistance genes (ARGs) was much higher than those (≤ 2.6％) of other samples.This study highlighted the significant impacts of drinking water treatment processes,transport pipe networks,and environmental risk exposure on viral guilds and functions,and provided a theoretical basis for optimizing operation and management of water supply systems.关键词
给水系统/病毒群落/潜在宿主/辅助代谢基因/抗生素抗性基因/宏基因组Key words
water supply system/viral community/potential hosts/auxiliary metabolic genes (AMGs)/antibiotic resistance genes (ARGs)/metagenomics引用本文复制引用
莫秋月,晏思岑,陈琪,陈天一,吴宗治,刘唐,陈倩,刘树枫..给水系统病毒群落及其潜在宿主和功能基因的分布特征[J].北京大学学报（自然科学版）,2024,60(4):701-712,12.基金项目
国家重点研发计划(2021YFA1202500)、国家自然科学基金(52100171和41907203)和中国博士后科学基金(2021T140010)资助 （2021YFA1202500）
