北京师范大学学报（自然科学版）2024，Vol.60Issue(3)：331-343,13.DOI:10.12202/j.0476-0301.2023229
线粒体分裂基因dnm1缺失对粟酒裂殖酵母细胞有丝分裂及能量代谢的影响
Deletion of dnm1(a mitochondrial division gene)affects mitosis and energy metabolism in Schizosaccharomyces pombe
摘要
Abstract
In Schizosaccharomyces pombe,the mitochondrial dynamin-related protein Dnm1 regulates dynamic mitochondrial fission/fusion.To examine the effect of dnm1 gene deletion on mitosis and energy metabolism in the fission yeast,live cells were imaged to analyze cell mitosis kinetics,RT-qPCR was performed to analyze transcription level of cdc2 and cdc13,high performance liquid chromatography-mass spectrometry was done to measure energy metabolites.The deletion of dnm1 gene led to slowed growth of fission yeast,microtubule bundles in dnm1Δ strain increased by(0.83±0.70)μm(P＜0.01)when compared to the wild-type in interphase,the number of strains producing 5 microtubule bundles increased,but the number of strains producing 3 microtubule bundles decreased.The spindle elongation time in dnm1Δ prolonged by(0.85±0.02)min(P＜0.05)in prophase,by(5.80±1.62)min(P＜0.01)in anaphase.Spindle elongation slowed down by 0.06 μm·min-1(P＜0.05)in anaphase.The dnm1Δstrain exhibited delayed spindle breakage and chromosome segregation.High-performance liquid chromatography-mass spectrometry revealed that dnm1Δ strain was deficient in coenzyme synthesis,resulting in a significant decrease in NADPH content(P＜0.05).The relative content of intermediate metabolites(glucose 6-phosphate,fructose 6-phosphate,citric acid,cis aconitic acid,pyruvate,isocitrate,and L-malic acid)were significantly reduced(P＜0.05),with major defect in ATP production.The cdc2 gene expression in logarithmic growth phase in dnm1Δ strain was significantly lower than the wild-type.关键词
裂殖酵母/线粒体/dnm1基因/有丝分裂/能量代谢Key words
fission yeast/mitochondria/dnm1 gene/mitosis/energy metabolism分类
生物学引用本文复制引用
张丁,余雯,刘梦楠,青昕,丁祥,侯怡铃..线粒体分裂基因dnm1缺失对粟酒裂殖酵母细胞有丝分裂及能量代谢的影响[J].北京师范大学学报（自然科学版）,2024,60(3):331-343,13.基金项目
四川省科技厅应用基础面上项目(2022NSFSC0107) （2022NSFSC0107）
四川省科技厅农业科技成果转化资金项目(2022NZZJ0003) （2022NZZJ0003）
四川省转移支付科技成果转移转化示范项目(22ZYZFSF0009 （）
23ZHSF0082). （）
