线粒体分裂基因dnm1缺失对粟酒裂殖酵母细胞有丝分裂及能量代谢的影响OA北大核心CSTPCD

In Schizosaccharomyces pombe,the mitochondrial dynamin-related protein Dnm1 regulates dynamic mitochondrial fission/fusion.To examine the effect of dnm1 gene deletion on mitosis and energy metabolism in the fission yeast,live cells were imaged to analyze cell mitosis kinetics,RT-qPCR was performed to analyze transcription level of cdc2 and cdc13,high performance liquid chromatography-mass spectrometry was done to measure energy metabolites.The deletion of dnm1 gene led to slowed growth of fission yeast,microtubule bundles in dnm1Δ strain increased by(0.83±0.70)μm(P＜0.01)when compared to the wild-type in interphase,the number of strains producing 5 microtubule bundles increased,but the number of strains producing 3 microtubule bundles decreased.The spindle elongation time in dnm1Δ prolonged by(0.85±0.02)min(P＜0.05)in prophase,by(5.80±1.62)min(P＜0.01)in anaphase.Spindle elongation slowed down by 0.06 μm·min-1(P＜0.05)in anaphase.The dnm1Δstrain exhibited delayed spindle breakage and chromosome segregation.High-performance liquid chromatography-mass spectrometry revealed that dnm1Δ strain was deficient in coenzyme synthesis,resulting in a significant decrease in NADPH content(P＜0.05).The relative content of intermediate metabolites(glucose 6-phosphate,fructose 6-phosphate,citric acid,cis aconitic acid,pyruvate,isocitrate,and L-malic acid)were significantly reduced(P＜0.05),with major defect in ATP production.The cdc2 gene expression in logarithmic growth phase in dnm1Δ strain was significantly lower than the wild-type.