北京师范大学学报（自然科学版）2024，Vol.60Issue(3)：358-366,9.DOI:10.12202/j.0476-0301.2023230
觉华岛近海水域人工鱼礁建设对日本蟳生物连通的潜在影响
Effect of artificial reefs construction on biological connectivity of Charybdis japonica in Juehua Island,Bohai Sea
摘要
Abstract
Artificial reefs improve the hydrodynamics at sea bottom,providing shelter and stepping stones for marine species,and facilitate fish attraction/production.In this work,10 pairs of microsatellite primers were selected to evaluate the effect of artificial reefs on genetic diversity and biological connectivity of Charybdis japonica in Juehua Island,Bohai Sea.Charybdis japonica samples were taken from five communities of seagrass-beds,two natural reefs,sand sediments,and artificial reefs ecosystem.The effective alleles Ne of the 10 loci was 2.94-17.72,the observed heterozygosity Ho and expected heterozygosity He were 0.30-0.82 and 0.67-0.95,respectively.Genetic diversity of Charybdis japonica from various ecosystems was high,the effective allele number Ne was 6.06-7.45,observed heterozygosity Ho ranged from 0.64 to 0.74,expected heterozygosity He was from 0.85 to 0.88.The mean Shannon information index was 1.95±0.07,and polymorphism information content PIC from all ecosystems was(80.03±1.31)％.Average F-statistics(FIS,FIT,FST)of the 10 microsatellite loci of Charybdis japonica were 0.207±0.156,0.240±0.152,and 0.050±0.013,respectively,indicating different inbreeding degree of genetic differentiation.FST values of Charybdis japonica among various ecosystems ranged from 0.001 to 0.026,the genetic differentiation in Charybdis japonica of all the paired ecosystems was small(FST＜0.05).The gene flow of Charybdis japonica was from 9.410 to 567.932,much greater than 1.The level of gene exchange in Charybdis japonica among different ecosystems was high,showing strong biological connectivity.The biological connectivity among seagrass-beds,natural reefs and artificial reefs was very high,but that of the sand sediment with other ecosystems was low.This verified that artificial reefs have beneficial effects for habitat restoration and resource conservation.关键词
微卫星DNA/日本蟳/遗传多样性/人工鱼礁/生物连通/觉华岛Key words
microsatellite DNA/Charybdis japonica/genetic diversity/artificial reefs/biological connectivity/Juehua Island分类
资源环境引用本文复制引用
张兆衡,武媛,孙涛,杨薇,张子玥..觉华岛近海水域人工鱼礁建设对日本蟳生物连通的潜在影响[J].北京师范大学学报（自然科学版）,2024,60(3):358-366,9.基金项目
国家重点研发计划重点专项资助项目(2018YFC1406404) （2018YFC1406404）
山东省重点研发计划(重大科技创新工程)资助项目(2021CXGC011201) （重大科技创新工程）
国家自然科学基金资助项目(52079006) （52079006）
