北京师范大学学报（自然科学版）2024，Vol.60Issue(3)：367-374,8.DOI:10.12202/j.0476-0301.2023214
氧化乐果降解过程中核心菌群构建及功能解析
Construction and functional analysis of the core microbial community for omethoate degradation
摘要
Abstract
Modem molecular biotechnology can analyze microbial communities further compared with traditional microbial research.Bioinformatics analysis of microbial community samples was conducted for domestication of garden soil with Omega,to explore the response law of soil microbial community structure to Omega,and to analyze the functions of core microbial communities.During domestication,the community was found to have completed a directional shift towards the degradation and adaptation of omethoate.As the degradation efficiency gradually improved,the degradation rate of omethoate reached over 90％at 24 days.From 18th to 24th days,the community structure reached a stable state at the phylum level,with the dominant phylum being Bacteroidetes,Proteobacteria,Acidobacteria and Gemmatimonadetes,the dominant communities at the genus level were Flavobacterium,Stenotrophobacter,Pseudomonas,Sphingomonas,Nitrospira.By modularizing the co-occurrence network and dividing the top three contributing modules into core functional bacterial communities,it was inferred that the core bacterial genera played a dominant role in community changes.Ferruginibacter was found to have contributed the greatest,Flavobacterium had the strongest inter group connectivity.Combined with PICRUSt2,functional predictions were made for the dominant communities and core functional bacterial communities at different stages of domestication.It was found that at the primary functional level,metabolic function was the most important,but at the secondary functional level,significant differences were present;these were mainly reflected in the high growth and passage levels and high secondary metabolite biosynthesis levels of core functional bacterial communities,to achieve complementary metabolic functions.关键词
高通量测序/氧化乐果/功能菌群/功能预测Key words
high-throughput sequencing/omethoate/functional microbiota/functional prediction分类
农业科学引用本文复制引用
宣元焱,孙寓姣,尹萌,赵娟娟..氧化乐果降解过程中核心菌群构建及功能解析[J].北京师范大学学报（自然科学版）,2024,60(3):367-374,8.基金项目
国家自然科学基金资助项目(51678054,51978056) （51678054,51978056）
