北京中医药大学学报2024，Vol.47Issue(7)：939-947,9.DOI:10.3969/j.issn.1006-2157.2024.07.009
人参-玉竹药对抑制炎性小体NLRP1、NLRC4、AIM2活化及调控炎症因子表达的抗抑郁作用机制研究
Study on the antidepressant mechanism of ginseng-fragrant solomonseal rhizome couplet medicines on inhibiting the activation of inflammasomes NLRP1,NLRC4,and AIM2,and regulating the expression of inflammatory cytokines
摘要
Abstract
Objective To observe the effect of Renshen(ginseng)-Yuzhu(fragrant solomonseal rhizome)on inflammatory factors and inflammasomes in depression rats,and to explore the antidepressant mechanism of Renshen-Yuzhu.Methods Fifty male SD rats were divided into the blank group,model group,fluoxetine group(2.1 mg/kg),Renshen-Yuzhu low-dose group(1.89 g/kg),and Renshen-Yuzhu high-dose group(5.67 g/kg),with ten rats in each group.Except for the blank group,the rats in the other groups were treated with chronic unpredictable mild stress to establish a depression rat model.On the second day after the end of modeling,the rats in each group were given the corresponding drugs once daily for 14 days.After modeling and dosing,body weight,forced swimming immobility time,and sucrose preference rate were measured.After dosing,hematoxylin-eosin staining was used to detect neuronal damage in the cerebral cortex,enzyme-linked immunosorbent assay was used to detect the contents of interleukin-4(IL-4),interleukin-23(IL-23),and interleukin-27(IL-27)in cortex,real-time PCR was used to detect the mRNA expression of interleukin-24(IL-24)in cortex,and the protein expressions of nucleotide-binding oligomerization domain-like receptor protein 1(NLRP1),absent in melanoma 2(AIM2),and nucleotide-binding oligomerization domain-like receptor protein 4(NLRC4)were detected by Western blotting.Results After dosing,compared with the blank group,the body weight of the model group decreased,the sucrose preference rate decreased,the swimming immobility time was prolonged,the neuronal tissue in cortex was destroyed,the content of IL-4 in cortex decreased,the contents of IL-23 and IL-27 in cortex increased,and the protein expressions of NLRP1,AIM2 and NLRC4 in cortex increased(P＜0.01).Compared with the model group,the body weight of rats in each administration group increased,the sucrose preference rate increased,the swimming immobility time was shortened,the damage of neuronal tissues in cortex improved,the content of IL-4 in cortex increased,the contents of IL-23 and IL-27 in cortex decreased,and the protein expressions of NLRP 1,AIM2 and NLRC4 in cortex decreased(P＜0.05).Conclusion Renshen-Yuzhu couplet medicines can improve the depressive-like behavior and exert antidepressant effect in chronic stress rats,and its mechanism may be related to the inhibition of NLRP 1,NLRC4,AIM2 inflammasome activation and its mediated inflammatory response in cortex,reducing pro-inflammatory cytokines,and increasing the level of antiinflammatory cytokines.关键词
抑郁症/人参/玉竹/炎症因子/炎性小体/大鼠Key words
depression/ginseng/fragrant solomonseal rhizome/inflammatory factors/inflammasomes/rats分类
医药卫生引用本文复制引用
张华宇,曹佳璐,郑冰元,刘倩,梁可,乔铁..人参-玉竹药对抑制炎性小体NLRP1、NLRC4、AIM2活化及调控炎症因子表达的抗抑郁作用机制研究[J].北京中医药大学学报,2024,47(7):939-947,9.基金项目
中国博士后科学基金资助项目(No.2021MD703843) （No.2021MD703843）
辽宁省自然科学基金项目(No.2021-BS-175) （No.2021-BS-175）
辽宁省高等学校创新人才支持计划(No.LR2019039) Postdoctoral Science Foundation of China(No.2021MD703843) （No.LR2019039）
