现代中医临床2024，Vol.31Issue(4)：14-18,5.DOI:10.3969/j.issn.2095-6606.2024.04.003
肾草酸钙结石湿热体质、痰湿体质与肠道菌群相关性的临床研究
Clinical study on the relationship between kidney calcium oxalate stones,damp-heat constitution,phlegm-dampness constitution and intestinal flora
摘要
Abstract
Objective To investigate the relationship between kidney calcium oxalate stones,damp-heat constitution,phlegm-dampness constitution,and intestinal flora.Methods 60 patients with recurrent renal calcium oxalate stones were divided into two groups:30 patients with a damp-heat constitution and 30 patients with a phlegm-dampness constitution.High-throughput sequencing of intestinal flora was performed,and 24-hour urine oxalic acid and citric acid levels were measured.Results The high-throughput sequencing of intestinal flora identified 3,790 operational taxonomic units(OTUs)across both groups.The damp-heat group had 2,394 OTUs,while the phlegm-dampness group had 2,289.Linear discriminant analysis revealed that the damp-heat group's flora was characterized by Proteobacteria C,Enterobacteriaceae,Enterobacteriales,and Proteobacteria,whereas the phlegm-dampness group's flora was characterized by Verrucomicrobiaceae,Bacteroides,Coprococcus,Proteobacteria A,and Clostridium teniculum.Comparative taxonomic analysis of OTU representative sequences showed increased abundance in the phlegm-dampness group at the phylum,class,and order levels,particularly Proteobacteria,Proteobacteria C,and Enterobacteriales.At the family level,Enterobacteriaceae and Peptostreptococcaceae were more abundant,while Verrucomicrobiaceae were significantly less abundant in the phlegm-dampness group.Genus-level analysis showed increased Escherichia and decreased Bacteroides abundance.Comparison of intestinal flora abundance indicated higher levels of Proteobacteria and Enterobacteriales in the phlegm-dampness group and higher levels of Firmicutes and Verrucomicrobiaceae in the damp-heat group,with statistically significant differences(P<0.05).Before treatment,24-hour urine oxalic acid and citric acid levels were higher in the damp-heat group than in the phlegm-dampness group(P<0.05).Post-treatment,the damp-heat group showed a significant reduction in oxalic acid levels(P<0.05),while the phlegm-dampness group showed a significant reduction in oxalic acid levels and an increase in citric acid levels(P<0.05).Conclusion There is a correlation between damp-heat constitution,phlegm-dampness constitution,and intestinal flora in patients with kidney calcium oxalate stones.Traditional Chinese Medicine(TCM)syndrome differentiation treatment can improve high-risk metabolic factors associated with kidney calcium oxalate stones.关键词
肾草酸钙结石/湿热体质/痰湿体质/肠道菌群/草酸/枸橼酸Key words
renal calcium oxalate calculus/dampness-heat constitution/phlegm-dampness constitution/intestinal flora/oxalic acid/citric acid分类
医药卫生引用本文复制引用
金振华,张继康,陈凯华,许坤,孙梦伟,卢子杰..肾草酸钙结石湿热体质、痰湿体质与肠道菌群相关性的临床研究[J].现代中医临床,2024,31(4):14-18,5.基金项目
江苏省中医药管理局(No.RC201902) （No.RC201902）
