草业科学2024，Vol.41Issue(6)：1384-1396,13.DOI:10.11829/j.issn.1001-0629.2023-0205
接种AMF对'阿坝'垂穗披碱草根系耐低温生理的影响
Effects of AMF inoculation on low-temperature tolerance physiology of Elymus nutans'Aba'roots
摘要
Abstract
In this study,we sought to determine the effects of arbuscular mycorrhizal fungi(AMF)on the low-temperature tolerance of Gramineae grasses using the fungus Glomus intraradices(GI)and grass Elymus nutans'Aba'.Pot-based experiments were conducted to investigate the effects of inoculation with GI on the root physiological characteristics of E.nutans'Aba'at low temperature(15 ℃/10 ℃,16 h/8 h,day/night).The results revealed that low temperature had no significant effects on the rate of mycorrhizal infection of E.nutans'Aba'.However,low temperature was found to promote increases in the contents of hydrogen peroxide(H2O2)and malondialdehyde(MDA)and reductions the rate of superoxide anion production(O2·-)in the root system of E.nutans'Aba',whereas GI inhibited the accumulation of H2O2 and MDA caused by low temperature,although had no significant effects on O2·-.Low temperature was also found to enhance the activities of superoxide dismutase(SOD),peroxidase(POD),and ascorbate peroxidase(APX),although caused a reduction in the activity of catalase(CAT).Moreover,the activities of SOD,POD,and APX in the inoculation group(LT-AMF)were significantly lower than those in the non-inoculation group(LT)(P＜0.05).Furthermore,low temperature promoted significant increases in the content of soluble protein(SP)and reductions in the content of betaine(Gb),although had no significant effects on the content of free proline(Pro).Inoculation with GI contributed to a significant reduction in the content of SP,although had no significant effects on the contents of Pro and Gb.In conclusion,by promoting the activities of antioxidant enzymes and reducing the intensity of osmotic stress,inoculation with G.intraradices can enhance the low-temperature tolerance of E.nutans'Aba'.关键词
丛枝菌根真菌/低温/禾本科/根系/活性氧类物质/抗氧化酶/渗透调节物质Key words
arbuscular mycorrhizal fungi/low temperature/Gramineae/root/reactive oxygen species/antioxidant enzyme/osmotic regulator引用本文复制引用
张海娟,周学丽,芦光新,金鑫,范月君..接种AMF对'阿坝'垂穗披碱草根系耐低温生理的影响[J].草业科学,2024,41(6):1384-1396,13.基金项目
青海省科技厅重点研发与转化计划"禾/豆混播的微生物调控及菌肥研发"项目(2022-SF-147) （2022-SF-147）
2021年度青海省"昆仑英才·高端创新创业人才"计划项目 （）
