草业科学2024，Vol.41Issue(6)：1429-1440,12.DOI:10.11829/j.issn.1001-0629.2022-0907
不同添加水平甜菊糖苷对绵羊体外产气参数及瘤胃发酵的影响
Effect of varying levels of stevioside supplementation on in vitro gas production and rumen fermentation in sheep
摘要
Abstract
We studied the effect of varying supplemental dietary stevioside levels on nutrient degradation and rumen fermentation in sheep.In this study,eight experimental sheep treatments were set up,with stevioside supplements at 0(CK group),0.01％,0.04％,0.07％,0.10％,0.15％,0.20％,and 0.30％(based on the air-dried matter in the diet),and the rumen fermentation and nutrient degradation rates were evaluated for 48 h by an in vitro gas production method.The results showed that the gas production(GP),dry matter degradation rate(DMD),volatile fatty acid,and methane yields,in all treatments,increased with prolonged rumen degradation time,peaking at 48 h.However,pH decreased with prolonged rumen degradation time.After 12 h,the GP of the 0.10％group showed a significant increase compared to the CK group(P＜0.05).At 24 h,the DMD in the 0.20％and 0.30％groups was significantly lower than in the CK group(P＜0.05),with reductions of 9.71％and 5.80％(P＜0.05),respectively.Compared with the CK group,there were no significant differences in DMD and crude protein degradation rate of all other groups at 48 h(P＞0.05).With an increase in fermentation time,the content of ammonium nitrogen(NH3-N)in the rumen fluid of each treatment ranged from 7.05～13.97 mg·dL-1,which was within the appropriate concentration range for rumen microbial activity.The 0.07％and 0.10％groups maintained elevated levels of acetic acid and total volatile fatty acids.In conclusion,the optimal concentration range of stevioside in sheep diet was observed to be 0.07％～0.10％.These results provide a theoretical basis for adopting the appropriate stevioside supplementation levels in sheep production practices.关键词
甜叶菊/甜味剂/体外发酵/模拟瘤胃/瘤胃养分降解/瘤胃发酵/甲烷Key words
Stevia rebaudiana/sugar substitute/in vitro fermentation/simulated rumen/rumen nutrient degradation/rumen fermentation/methane引用本文复制引用
齐帅,焦婷,李雄雄,李淑艳,王虎宁,沙玉柱,赵生国..不同添加水平甜菊糖苷对绵羊体外产气参数及瘤胃发酵的影响[J].草业科学,2024,41(6):1429-1440,12.基金项目
甘肃省高校创新基金项目(2022B-096) （2022B-096）
甘肃省科技计划(项目技术传销引导计划-鲁甘科技协作专题)项目(22CX2NA005) （项目技术传销引导计划-鲁甘科技协作专题）
南疆重点产业创新发展支撑计划项目(2022DB017) （2022DB017）
甘肃省教育厅研究生"创新之星"项目(2023CXZX-665) （2023CXZX-665）
