小麦及其祖先物种Hsp70基因家族鉴定与表达分析OA北大核心CSTPCD

Heat shock protein 70 (Hsp70) exerts a crucial influence on plant development and response to biotic and abiotic stresses. In order to explore the evolutionary relationships,functions and expression patterns of the Hsp70 gene family in wheat (Triticum aestivum),this study conducted a comprehensive bioinformatics analysis of the Hsp70 gene family in Triticum urartu,Aegilops speltoides,Triticum turgidum,Aegilops tauschii and common wheat,and the expression patterns of some Hsp70 genes under different exogenous hormones and environmental stress were analyzed by RT-qPCR. The results showed that,respectively,from the five species,30,41,60,28 and 94 Hsp70 genes were identified. Phylogenetic analysis showed that the members of the Hsp70 family in the five species were divided into 5 subfamily groups,and the number of members in each group was not equal. Most of the members are distributed in Group I,and most of the Hsp70 members in the same subfamily have similar gene structures and conserved motifs. Further comprehensive analysis of chromosome localization and repetition events of the Hsp70 gene in the five species revealed that the Hsp70 gene was unevenly distributed on each of the chromosomes,and a total of 12 tandem repeat events and 110 fragment replication events were found from the five species,indicating that fragment replication events promoted the expansion of the Hsp70 gene family in wheat. The analysis of cis-acting elements showed that there were various light response elements,stress response elements,hormone response elements and growth as well as development regulatory elements in the promoter region of Hsp70 gene in the five wheat species. In addition,RT-qPCR results indicated that particular Hsp70 genes in the five wheat species had varying degrees of response under different hormone treatments and stress. The expression of eight Hsp70 genes was up-regulated under high temperature and drought stress. The identification of the Hsp70 gene in wheat and its ancestral species and elucidation of its evolution provides a theoretical basis for further research on the function of the Hsp70 gene in wheat growth and development,as well as its response mechanism under stress.