草业学报2024，Vol.33Issue(7)：68-83,16.DOI:10.11686/cyxb2023335
盐生草AKR基因家族成员的鉴定及根系盐胁迫响应基因HgAKR42639的耐盐分析
Identification of AKR gene family members in Halogeton glomeratus and salt tolerance analysis of the root salt stress response gene HgAKR42639
摘要
Abstract
In order to explore the biological function of the AKR gene family and the salt tolerance of the root salt stress response gene HgAKR42639 in Halogeton glomeratus,the aldo-keto reductase gene (AKRs) was identified based on full-length transcriptome sequencing of H. glomeratus,and the protein sequence encoded by AKRs in H. glomeratus was analyzed by bioinformatics methods. The target gene expression,physiological indexes and sodium and potassium content in roots of H. glomeratus and the Arabidopsis thaliana HgAKR42639 gene were measured under 200 mmol·L-1 NaCl stress for 0,6,12,24 and 48 hours. We found that 23 HgAKRs were identified from the H. glomeratus transcriptome,encoding amino acid sequences ranging from 165 to 664 aa,and the prediction of subcellular localization was mainly in the cytoplasm. AKR protein conserved domains were highly similar with 3 motifs,and there were core elements,enhancing elements and stress response elements in the analysis of promoter cis-acting elements. The results of qRT-PCR showed that the relative expression of HgAKR42639 increased initially and then decreased in both H. glomeratus roots and A. thaliana,and the expression of HgAKR42639 peaked at 24 hours. With increase in exposure time to salt stress,the activities of catalase (CAT),peroxidase (POD),superoxide dismutase (SOD) and the proline content showed an increasing trend,while the contents of malondialdehyde (MDA) and soluble protein showed a downward trend,reaching their lowest levels at 24 hours. The content of Na+and K+decreased under the salt stress treatment for 24 hours,and K+/Na+reached the maximum value. In summary,the AKR family genes of H. glomeratus were obtained in this study,and the results provide a theoretical basis for further research on the molecular mechanism of regulation of response to salt stress in AKR genes in H. glomeratus. This research provides a foundation for further verification of the HgAKR42639 gene role in salt tolerance.关键词
盐生草/AKR基因家族/生物信息学分析/盐胁迫Key words
Halogeton glomeratus/AKR gene family/bioinformatics analysis/salt stress引用本文复制引用
张震欢,李葆春,姚立蓉,汪军成,司二静,张宏,杨轲,马小乐,孟亚雄,王化俊..盐生草AKR基因家族成员的鉴定及根系盐胁迫响应基因HgAKR42639的耐盐分析[J].草业学报,2024,33(7):68-83,16.基金项目
国家自然科学基金项目(31960072,32001514),财政部和农业农村部:国家现代农业产业技术体系项目(CARS-05-04B-2),甘肃省教育厅:产业支撑计划项目(2021CYZC-12),甘肃省青年基金项目(22JR5A880),甘肃省自然基金项目(20JR10RA507),甘肃农业大学伏羲青年英才计划(Ganfx-04Y11,Ganfx-03Y06)和甘肃农业大学国家级大学生创新创业训练计划重点支持领域项目(202110733001)资助. （31960072,32001514）
