彝医药金薄清咽颗粒制备工艺及对慢性咽炎模型大鼠的疗效机制OACSTPCD

Objective:To study the preparation technology of Yi medicine JinBo Qingyan Granules(JBQYG)and its therapeutic mechanism on chronic pharyngitis(CP)model rats.Methods:Selected the optimal excipients for molding process screening and HPLC analysis.JBQYG was used to intervene CP rats.The morphology,pathology,transcription factor p65(NF-κB p65)protein expression,inflammation and oxidation status of throat levels were detected.Results:The optimal excipient was lactose,and the molding technology was as follows:pure extract:lactose 8∶1,ethanol concentration 95%,ethanol dosage 10%,paeoniflorin was stable.JBQYG decreased the phosphorylation level of NF-κB p65 protein in throat of CP rats and increased the total antioxidant capacity,reversed the inflammation and oxidative stress induced by ammonia,thus improved the appearance and pathology of throat.JBQYG improved the oxidative stress more effectively than aspirin,the difference was statistically significant(P＜0.05).Conclusion:The formulation preparation process determined through the research is reasonable.JBQYG can improve the inflammatory and oxidative state,appearance and pathology of CP by decreasing the phosphorylation level of NF-κB p65 and increasing the total antioxidant capacity.