川北医学院学报2024，Vol.39Issue(8)：1014-1017,4.DOI:10.3969/j.issn.1005-3697.2024.08.002
JS-K对HBV阳性肝癌鸡胚移植瘤生长影响的实验研究
Experimental study on the impact of JS-K on the growth of HBV-positive HCC in transplanted chicken embryo tumors
摘要
Abstract
Objective:To explore the impact of the nitric oxide donor JS-K on tumor cell growth within a hepatitis B virus(HBV)-positive liver cancer model using chicken embryo transplantation.Methods:HepG2.2.15 cells,an HBV-positive liver cancer cell line,were inoculated into fertilized chicken embryos at 9 days of age.2 days post-inoculation,treatments were initiated,dividing the experiment into five groups:control group,JS-K 1 μg group,JS-K 5 μg group,JS-K 10 µg group,and sorafenib group(400 µg/piece)according to different processing methods,3 subjects in each group.Treatments were administered directly onto the tumors daily for 8 consecutive days,once a day.Post-treatment,tumors were excised for analysis.The size of each tumor was measured,photographed,and weighed,and the tumor inhibition rate was calculated.The effect of JS-K on tumor cells was evaluated using hematoxylin and eosin(HE)staining,and the expression of the cell migration-related protein Vimentin and N-Cadherin in tumor tissue were analyzed by Western blotting.Results:Compared with the control group,tumor volumes in the chicken embryos were reduced following treatment with varying doses of JS-K,with the 10 μg group showing the smallest tumor size.HE staining indicated a significant reduction in tumor cell counts post JS-K treatment,the expression level of Vimentin in the 1 µg JS-K group of chicken embryo transplant tumors was higher than that of the other groups,while the expression of N-cadherin was lower than that of the other groups.Conclusion:JS-K demonstrates a tumor-suppressive effect in HBV-positive liver cancer in a chicken embryo transplant model,which may be related to inhibiting the migration of tumor cells.关键词
乙型肝炎病毒/肝癌/JS-K/鸡胚/肿瘤迁移Key words
Hepatitis B virus/Hepatocellular carcinoma/JS-K/Chicken embryo/Migration分类
医药卫生引用本文复制引用
廖晏蔺,刘正芸,文娟,陈国丽,于万,王欢..JS-K对HBV阳性肝癌鸡胚移植瘤生长影响的实验研究[J].川北医学院学报,2024,39(8):1014-1017,4.基金项目
贵州省科技厅资助项目[黔科合基础(2020)1Y349 （2020）
贵州省教育厅资助项目[黔教技(2022)027号] （2022）
