川北医学院学报2024，Vol.39Issue(8)：1018-1021,1035,5.DOI:10.3969/j.issn.1005-3697.2024.08.003
郑怀贤经验方对股骨骨不连大鼠骨折恢复的影响
Effects of Zheng Huaixian's empirical formula on the recovery of femoral bone nonunion in rats
摘要
Abstract
Objective:To explore the effects of Zheng Huaixian's empirical formula on the recovery of femoral bone nonunion in rats.Methods:The femoral shafts were surgically severed to construct models of rat with femoral bone nonunion,and they were divided into model group and experimental group according to different processing methods.The healthy rats were enrolled as blank group.At 8 weeks after surgery,experimental group was given Zheng Huaixian's empirical formula(2.7 g/kg)for external application on affected limbs for 8 weeks,while model group and blank group were not treated.The changes of rat activities,hair color,defects of femoral shaft,pathological situations and serological indexes were observed.Results:In blank group,rats were active,appetite was normal,clothing hair was compact and glossy,bone structure of femoral shaft was normal,and arrangement of chondrocytes was ordered.Compared with model group,reduced activity state,appetite,hair dryness and dark color were improved in experimental group.There was bone callus at femoral defect site after 4 weeks of intervention,bone defect line was disappeared,bone cortex was continuous,arrangement disorder and structure lack of chondrocytes were improved after 8 weeks of intervention in experimental group.The levels of serum bone alkaline phosphatase(BALP),osteocalcin(OC),type Ⅰ procollagen N-terminal propeptide(PINP),transforming growth factor-β(TGF-β),se-rum vascular endothelial growth factor(VEGF)and bone morphogenetic protein-2(BMP-2)in experimental group were higher than those in model group(P＜0.05).Conclusion:Zheng Huaixian's empirical formula can improve osteoblasts activity,increase levels of TGF-β,VEGF and BMP,and promote fracture recovery in rats with femoral bone nonunion.关键词
骨不连/郑怀贤经验方/成骨细胞活性/转化生长因子β/血管内皮细胞生长因子/骨形态发生蛋白2Key words
Bone nonunion/Zheng Huaixian's empirical formula/Osteoblast activity/TGF-β/VEGF/BMP分类
医药卫生引用本文复制引用
魏林,檀亚军,孙玉,王金涛,叶锐彬..郑怀贤经验方对股骨骨不连大鼠骨折恢复的影响[J].川北医学院学报,2024,39(8):1018-1021,1035,5.基金项目
四川省中医药管理局科学技术研究专项(2021MS245) （2021MS245）
