长春中医药大学学报2024，Vol.40Issue(7)：746-750,5.DOI:10.13463/j.cnki.cczyy.2024.07.009
circ_0013958/miR-545轴对子宫内膜癌细胞RL-95-2增殖、凋亡和迁移的影响
Effect of circ_0013958/miR-545 axis on the proliferation,apoptosis and migration of endometrial cancer cell RL-95-2
摘要
Abstract
Objective To explore the effects of circ_0013958 on the proliferation,apoptosis and migration of endometrial cancer cell RL-95-2 and its possible mechanism.Methods Endometrial cancer tissues and paracancerous tissues of 51 cases were collected,and the expression levels of circ_0013958 and miR-545 in the tissues were detected by quantitative real time polymerase chain reaction(qRT-PCR).RL-95-2 cells were cultured in vitro and transfected with sh-circ_0013958,miR-545 mimics,si-circ_0013958 and miR-545 inhibitor respectively,and then cell proliferation,apoptosis,and migration were detected by CCK-8,flow cytometry,and Transwell assays,respectively.The levels of apoptosis-related proteins were detected by Western blot.The targeting relationship between circ_0013958 and miR-545 was verified by the dual luciferase reporter assay.Results Compared with paracancerous tissues,the expression level of circ_0013958 in endometrial cancer tissues was higher(P＜0.05),and the expression level of miR-545 was lower(P＜0.05).After knocking down circ_0013958 or up-regulating miR-545,the proliferation and migration ability of RL-95-2 cells was inhibited,while cell apoptosis was increased(P＜0.05).circ_0013958 targeted negative regulation of miR-545.Down-regulation of miR-545 reversed the anti-proliferation,anti-migration and pro-apoptosis effects mediated by knocking down circ_0013958.Conclusion circ_0013958 may promote the proliferation and migration of endometrial cancer RL-95-2 cells and hinder cell apoptosis by targeting down-regulation of miR-545.关键词
子宫内膜癌/circ_0013958/miR-545/细胞增殖/凋亡/迁移Key words
endometrial cancer/circ_0013958/miR-545/cell proliferation/apoptosis/migration分类
医药卫生引用本文复制引用
张仕田,何翔,杨帆,赵红利,龚钿..circ_0013958/miR-545轴对子宫内膜癌细胞RL-95-2增殖、凋亡和迁移的影响[J].长春中医药大学学报,2024,40(7):746-750,5.基金项目
四川省科技计划项目(2021YJ0137) （2021YJ0137）
