大豆科学2024，Vol.43Issue(4)：421-430,10.DOI:10.11861/j.issn.1000-9841.2024.04.0421
模拟干旱胁迫下大豆耐旱突变体的生理和转录组分析
Physiological and Transcriptomic Analysis of A Soybean Drought-tolerant Mutant Under Simulate Drought Stress
摘要
Abstract
In order to identify the key genes responsive to drought stress and to elucidate the underlying mechanism in drought tolerance of soybean seedlings,Zhechun 3 and its mutant Gm-lpa-ZC-2(ZC-2)were test in this research.The phenotype,relevant physiological and biochemical indexes were observed and determined after stress treatment under 20%PEG6000,and RNA-seq technology used for transcriptomic analysis.The results showed that the relative water content,the maximum quantum yield of PSII(Fv/Fm),soluble sugar content and soluble protein content of the mutant ZC-2 were significantly higher than those of Zhechun 3,and relative electrolyte leakage rate(REC)and malondialdehyde(MDA)content were lower than those of Zhechun 3,which could indicate that the mutant ZC-2 was more drought tolerant than Zhechun 3.The transcriptomic data indicated that there were totally 7 879 differentially expressed genes(DEGs)between Zhechun 3 and ZC-2,including 4 208 up-regulated genes and 3 671 down-regulated genes.There were 571 differential genes expressed on both day 2 and day 4 under treatment.In addition,the 571 DEGs were involved in 84 metabolic pathways.KEGG enrichment analysis indicated that DEGs were mainly enriched in pathways such as photosynthesis-antenna proteins,galactose metabolism,protein processing in endoplasmic reticulum,and flavonoid biosynthesis.Moreover,genes related to photosynthesis and sugar metabolism pathway as well as gene associated with drought stress,including TIFY10A,ZAT10,RD22,PM19L and XTH6,were detected.This study provided a foundation for mining candidate genes related to drought tolerance in soybean seedlings and studying the mechanism of soybean drought tolerance.关键词
大豆/干旱胁迫/PEG/苗期/生理指标/转录组Key words
soybean/drought stress/PEG/seedling/physiological indicators/transcriptome引用本文复制引用
曹颖,竹龙鸣,邓平,陈紫芸,袁凤杰..模拟干旱胁迫下大豆耐旱突变体的生理和转录组分析[J].大豆科学,2024,43(4):421-430,10.基金项目
浙江省农业(旱粮新品种选育)新品种选育重大科技专项(2021C02064-5). （旱粮新品种选育）
