东北地区大豆种质资源萌发期耐盐性鉴定OA北大核心CSTPCD

In order to clarify the salt tolerance characteristics of soybean germplasm resources in northeast China,and to provide excellent parents for the selection and breeding of salt-tolerant varieties of soybeans,this study took 348 soybean germplasm resources mainly from northeast China as test materials.We cultivated them in an artificial greenhouse,and selected them to be subjected to a low-salt stress treatment with 150 mmol·L-1 NaCl during the germination period,and then measured the germination potential,the germination rate,the shoot length and the root length.We calculated the relative salt damage rate and determined the salt tolerance grade and carried out the evaluation of salt tolerance based on the germination rate.The germination potential,shoot length and root length were measured,and the relative salt damage rate was calculated based on the germination rate,and the salt tolerance level was evaluated.89 highly tolerant materials were then subjected to high salt stress with 210 mmol·L-1 NaCl solution in order to screen out the materials with stable salt tolerance during the germination period.The results showed that the measured values of all indexes under salt stress decreased significantly compared with the control,and there was a significant or highly significant relationship between the indexes.According to the relative salt damage rate,the salt tolerance was evaluated,89 highly tolerant materials were screened under low salt stress,and 33 salt-tolerant soybean materials were screened under high salt stress.The 33 soybean materials screened in this study can provide important basic materials for breeding salt-tolerant soybean varieties and analyzing the mechanism of salt tolerance in soybean.