大连工业大学学报2024，Vol.43Issue(4)：251-256,6.DOI:10.19670/j.cnki.dlgydxxb.2024.0403
利用差异转录组定位Paraburkholderia caffeinilytica CF1咖啡因降解功能基因
Using differential transcriptome to located functional gene of Paraburkholderia caffeinilytica CF1
摘要
Abstract
An efficient caffeine-degrading strain Paraburkholderia caffeinilytica CF1 isolated from a tea garden in China.To identify genes involved in caffeine degradation,a global transcription analysis of strain CF1 growing with and without caffeine addition were carried out and differential transcriptome analysis under the two conditions were conducted.The results showed that 4 871 Unigene sequences were obtained and 96.96％of the obtained Unigene sequences could be annotated according to the whole genome sequence of strain CF1.The difference gene analysis between groups reveals that 123 genes were up-regulated and 2 669 genes were down-regulated with caffeine addition.Among the up-regulated genes,112 genes were encoded on the chromosome and 11 genes arranged side by side were encoded on the giant plasmid.By analyzing the sequence alignment and activities of key enzymes,the redundant gene cluster containing the 11 up-regulated genes of caffeine degradation was elucidated.关键词
咖啡因降解/转录组测序/差异转录组分析Key words
caffeine degradation/RNA-seq/differential transcriptome analysis分类
生物科学引用本文复制引用
陈森,高婕,王剑桥,高子晴..利用差异转录组定位Paraburkholderia caffeinilytica CF1咖啡因降解功能基因[J].大连工业大学学报,2024,43(4):251-256,6.基金项目
广东省自然科学基金项目(2020A1515110189). （2020A1515110189）
