大连工业大学学报2024，Vol.43Issue(4)：257-260,4.DOI:10.19670/j.cnki.dlgydxxb.2024.0404
产L-阿拉伯糖异构酶基因工程菌的构建及优化
Construction and optimization of genetically engineered bacteria producing L-arabinose isomerase
摘要
Abstract
L-arabinose isomerase(L-AI)can specifically act on the substrate D-galactose to produce D-tagatose.To obtain a high-yield L-AI strain,the L-AI coding gene araA gene derived from L.brevis sp.D-tag1 was connected to pET-30a(+)expression plasmid to construct a recombinant plasmid.Then the recombinant plasmid was transferred into E.coli BL21(DE3)by heat shock method,and E.coli BL21(DE3)/pET-30a(+)-araA was constructed.The codon optimization of araA gene was carried out according to the codon preference of host E.coil.The optimized araAo gene was connected to pET-30a(+),then transferred into E.coli BL21(DE3)to construct E.coli BL21(DE3)/pET-30a(+)-araAo.The results showed the optimized strain showed a significant increase in expression levels compared to the unoptimized strain,with an increase in enzyme activity of 79.2％.关键词
L-阿拉伯糖异构酶/D-塔格糖/密码子优化Key words
L-arabinose isomerase/D-tagatose/codon optimization分类
生物科学引用本文复制引用
张艳芳,王晓茹,张一帆,张春枝..产L-阿拉伯糖异构酶基因工程菌的构建及优化[J].大连工业大学学报,2024,43(4):257-260,4.基金项目
辽宁省教育厅科学研究经费项目(面上项目)(LJKZ0514). （面上项目）
