南方医科大学学报2024，Vol.44Issue(7)：1297-1305,9.DOI:10.12122/j.issn.1673-4254.2024.07.09
异功散通过调控肠道微生物改善痴呆症模型大鼠的认知下降
Yigong San improves cognitive decline in a rat model of Alzheimer's disease by regulating intestinal microorganisms
摘要
Abstract
Objective To investigate the effect of Yigong San(YGS)on learning and memory abilities of rats with lipopolysaccharide(LPS)-induced cognitive decline and explore its possible mechanism in light of intestinal microbiota.Methods Forty SD rats were randomly divided into control group,model group,donepezil(1.3 mg/kg)group,and high-dose(5.25 g/kg)and low-dose(2.63 g/kg)YGS treatment groups.After 24 days of treatment with the corresponding drugs or water by gavage,the rats in the latter 4 groups received an intraperitoneal injection of LPS(0.5 mg/kg)to establish models of Alzheimer's disease(AD).Water maze test and HE staining were used to evaluate the changes in learning and memory abilities and pathomorphology of the hippocampus.The changes in gut microbial species of the rats were analyzed with 16S rRNA sequencing,and the levels of IL-6,TNF-α,and IL-1β in the brain tissue and serum were detected using ELISA.Results Compared with the AD model group,the YGS-treated rats showed significantly shortened escape latency on day 5 after modeling,reduced neuronal degeneration and necrosis in the hippocampus,lowered pathological score of cell damage,and decreased levels IL-6,TNF-α and IL-1β in the brain tissue and serum.The YGS-treated rats showed also obvious reduction of Alpha diversity indicators(ACE and Chao1)of intestinal microbiota with significantly increased abundance of Prevotellaceae species at the family level and decreased abundance of Desulfovibrionaceae,which were involved in such metabolic signaling pathways as cell community prokaryotes,membrane transport,and energy metabolism.Conclusion YGS improves learning and memory abilities and hippocampal pathomorphology in AD rat models possibly by regulating the abundance of intestinal microbial species such as Prevotellaceae to affect the metabolic pathways for signal transduction,cofactors,and vitamin metabolism.关键词
异功散/脂多糖/认知下降/肠道微生物Key words
Yigong San/lipopolysaccharide/cognitive decline/intestinal microbiota引用本文复制引用
曾静,陈荣,任香怡,花雷,阳勇,魏江平,张小梅..异功散通过调控肠道微生物改善痴呆症模型大鼠的认知下降[J].南方医科大学学报,2024,44(7):1297-1305,9.基金项目
重庆市技术创新与应用发展专项重点项目(cstc2021jscx-dxwtBX0012) （cstc2021jscx-dxwtBX0012）
重庆市自然科学基金项目博士后项目(cstc2021jscx-bshX0054) （cstc2021jscx-bshX0054）
重庆市中药研究院基本科研业务费项目(jbky20210003) （jbky20210003）
湖南省卫健委一般指导课题项目(D202313058007) （D202313058007）
