南方医科大学学报2024，Vol.44Issue(7)：1336-1344,9.DOI:10.12122/j.issn.1673-4254.2024.07.13
金缕半枫荷的水提取物抑制胰腺癌的作用机制:活性成分、关键靶点和信号通路
Therapeutic mechanism of aqueous extract of Semiliquidambar cathayensis Chang root for pancreatic cancer:the active components,therapeutic targets and pathways
摘要
Abstract
Objective To explore the key targets and signaling pathways in the therapeutic mechanism of Semiliquidambar cathayensis Chang(SC)root against pancreatic cancer network pharmacology and molecular docking studies and cell experiments.Methods The targets of SC and pancreatic cancer were predicted using the network pharmacological database,the protein-protein interaction network was constructed,and pathways,functional enrichment and molecular docking analyses were performed.CCK-8 assay was used to test the inhibitory effect of the aqueous extract of SC root on 8 cancer cell lines,and its effects on invasion,migration,proliferation,and apoptosis of pancreatic cancer cells were evaluated.Western blotting was performed to verify the results of network pharmacology analysis.Results We identified a total of 18 active components in SC,which regulated 21 potential key targets in pancreatic cancer.GO and KEGG pathway enrichment analyses showed that these targets were involved mainly in the biological processes including protein phosphorylation,signal transduction,and apoptosis and participated in cancer signaling and PI3K-Akt signaling pathways.Among the 8 cancer cell lines,The aqueous extract of SC root produced the most obvious inhibitory effect in pancreatic cancer cells,and significantly inhibited the invasion,migration,and proliferation and promoted apoptosis of pancreatic cancer Panc-1 cells(P<0.05).Western blotting confirmed that SC significantly inhibited the phosphorylation levels of PI3K and AKT in Panc-1 cells(P<0.001).Conclusion The therapeutic effect of SC root against pancreatic cancer effects is mediated by its multiple components that act on different targets and pathways including the PI3K-Akt pathway.关键词
金缕半枫荷/胰腺癌/网络药理学/分子对接/通路分析Key words
Semiliquidambar cathayensis Chang/pancreatic cancer/network pharmacology/molecular docking/pathway analysis引用本文复制引用
黄燕,覃璐璐,管少兴,管宴萍,韦玉茹,操艾伶,李冬梅,韦桂宁,苏启表..金缕半枫荷的水提取物抑制胰腺癌的作用机制:活性成分、关键靶点和信号通路[J].南方医科大学学报,2024,44(7):1336-1344,9.基金项目
国家自然科学基金(81703769) （81703769）
广西人才基地项目(AD22035055) （AD22035055）
广东省自然科学基金(2019A1515011669) Supported by National Natural Science Foundation of China(81703769). （2019A1515011669）
