风湿病与关节炎2024，Vol.13Issue(7)：16-21,6.DOI:10.3969/j.issn.2095-4174.2024.07.004
云克对类风湿关节炎患者骨代谢及Wnt/DKK1相关因子表达研究
Effect of Tc-MDP on Bone Metabolism and Wnt/DKK1 Related Factor Expression in RA Patients
摘要
Abstract
Objective:To observe the therapeutic effect and safety of Tc-MDP on inflammation mediated bone metabolism in RA(rheumatoid arthritis)patients,as well as its impact on Wnt/DKK1 signaling pathway related proteins.Methods:Sixty RA patients who visited the Rheumatology and Immunology Department of the Second Affiliated Hospital of Guizhou University of Chinese Medicine from November 2020 to December 2021 were selected as the study subjects.They were randomly divided into a treatment group and a control group using a random number table method,with 30 patients in each group.The control group orally took methotrexate tablets combined with leflunomide tablets,while the treatment group were treated with Tc-MDP(trade name:Yunke)based on the treatment for the control group.Bone density was measured before treatment and at 3,6,and 12 months after treatment to observe the percentage change in bone density.Wnt/β-catenin related factors(β-catenin,DKK1,lLRP6),cytokines(IL-17,IL-6)and bone metabolism markers(β-CTX,tTRAP5b)were measured before treatment and at 12 weeks after treatment.Results:The ESR and DAS28-ESR scores,CRP,HAQ,and VAS scores of the treatment group decreased from week 4 to week 12,and the decrease was more significant compared with the control group(P<0.05)②After 12 weeks of treatment,the serum levels of bone destruction markers,inflammatory factors,and Wnt signaling pathway inhibitor DKK1 in the treatment group were lower than those in the control group(P<0.05),while the serum levels of Wnt pathway activator β-catenin and LRP6 in the treatment group were higher than those in the control group(P<0.05).③ After treatment,there was no statistically significant difference in the percentage change of spinal and hip bone density between the two groups(P>0.05).The percentage changes in hip and spinal bone density in both groups increased significantly in the 12th and 3rd months(P<0.05).Conclusion:Yunke can improve the clinical symptoms of RA patients,reduce disease activity,regulate bone metabolism,and may mediate the progression of bone destruction in RA patients through the Wnt/DKK1 signaling pathway,with good safety.关键词
类风湿关节炎/云克/Wnt/DKK1/骨代谢/随机对照试验Key words
rheumatoid arthritis/Yunke/Wnt/DKK1/bone metabolism/randomized controlled trial引用本文复制引用
唐芳,蒋总,秦瑶,马武开,兰维娅,刘正奇..云克对类风湿关节炎患者骨代谢及Wnt/DKK1相关因子表达研究[J].风湿病与关节炎,2024,13(7):16-21,6.基金项目
国家自然科学基金项目(82160917) （82160917）
贵州省卫生健康委科学技术基金项目(gzwkj2021-144,gzwkj2021-140,gzwkj2021-142,gzwkj2021-143) （gzwkj2021-144,gzwkj2021-140,gzwkj2021-142,gzwkj2021-143）
贵州省中医药管理局中医药、民族医药科学技术研究项目(QZYY-2020-005,QZYYXG-2021-7) （QZYY-2020-005,QZYYXG-2021-7）
贵州省基础研究(自然科学)项目(黔科合基础-ZK[2023]一般435,黔科合基础-ZK[2023]一般436) （自然科学）
贵州省中医风湿免疫疾病临床研究中心(黔科合平台人才[2020]2202号) （黔科合平台人才[2020]2202号）
贵州中医药大学第二附属医院院内科研项目(GZEYK-Y[2021]2号) （GZEYK-Y[2021]2号）
贵州省大学生创新创业训练计划项目(202110662031) （202110662031）
