分析化学2024，Vol.52Issue(6)：763-777,15.DOI:10.19756/j.issn.0253-3820.231273
仿生印迹亲和分析技术研究进展
Research Progress of Biomimetic Imprinting Affinity Analysis Technique
摘要
Abstract
Molecular imprinting is a biomimetic technique that simulates the specific recognition of biological macromolecules such as antibody. Based on molecular imprinting and high-specificity affinity analysis,the biomimetic imprinting affinity analysis (BIA) possesses many advantages such as high sensitivity,strong tolerance,good specificity and low cost,and thus,it has shown excellent prospects in food safety detection,pharmaceutical analysis and environmental pollution monitoring. In this review,the construction methods of recognition interfaces for BIA were summarized,including bulk polymerization,electro-polymerization and surface molecular imprinting. The application of molecularly imprinted polymers in different analysis methods,such as radiolabeled affinity analysis,enzyme-labeled affinity analysis,fluorescence-labeled affinity analysis,chemiluminescence affinity analysis and electrochemical immunosensor was mainly discussed. Furthermore,the challenges and future development trends of BIA in practical application were elucidated. This review might provide new reference ideas and technical supports for the further development of BIA technique.关键词
分子印迹/仿生/亲和分析/评述Key words
Molecularly imprinted/Biomimetic technology/Affinity analysis/Review引用本文复制引用
李兆周,袁云霞,韦玉花,张孝冲,陈秀金,王耀,牛华伟,李芳,高红丽,于慧春..仿生印迹亲和分析技术研究进展[J].分析化学,2024,52(6):763-777,15.基金项目
洛阳市公益性行业科研专项项目(No.2202021A)、河南省优秀青年科学基金项目(No.202300410121)、国家自然科学基金项目(Nos.31701694,U1504330,31702218)、河南省青年人才托举工程项目(No.2020HYTP029)和河南省研究生教育改革与质量提升工程项目(No.HNYJS2020JD06)资助.Supported by the Special Fund for Scientific Research in the Public Interest from Luoyang City,Henan Province,China(No.2202021A),the Natural Science Foundation of Henan Province,China(No.202300410121),the National Natural Science Foundation of China(Nos.31701694,U1504330,31702218),the Youth Talent Support Project of Henan Province,China(No.2020HYTP029)and the Postgraduate Education Reform and Quality Improvement Project of Henan Province,China(No.HNYJS2020JD06). （No.2202021A）
