仿生印迹亲和分析技术研究进展OA北大核心CSTPCD
Research Progress of Biomimetic Imprinting Affinity Analysis Technique
分子印迹是一种模拟生物分子特异性识别的仿生技术,通过制备分子印迹聚合物(MIPs),结合高特异性亲和分析方法所构建的仿生印迹亲和分析(Biomimetic imprinting affinity analysis,BIA)技术具有灵敏度高、耐受性强、专一性好以及成本低等优势,在食品安全检测、药物分析和环境污染监测等领域展现出良好的发展潜力.本文首先介绍了BIA识别界面的构建方法,包括本体聚合、电聚合和表面印迹法等,总结了近年来MIPs在放射标记亲和分析、酶标记亲和分析、荧光标记亲和分析、化学发光亲和分析和电化学免疫传感等不同分析方法中的研究和应用进展,最后讨论了该技术在实际应用中所面临的挑战和未来的发展趋势.
Molecular imprinting is a biomimetic technique that simulates the specific recognition of biological macromolecules such as antibody. Based on molecular imprinting and high-specificity affinity analysis,the biomimetic imprinting affinity analysis (BIA) possesses many advantages such as high sensitivity,strong tolerance,good specificity and low cost,and thus,it has shown excellent prospects in food safety detection,pharmaceutical analysis and environmental pollution monitoring. In this review,the construction methods of recognition interfaces for BIA were summarized,including bulk polymerization,electro-polymerization and surface molecular imprinting. The application of molecularly imprinted polymers in different analysis methods,such as radiolabeled affinity analysis,enzyme-labeled affinity analysis,fluorescence-labeled affinity analysis,chemiluminescence affinity analysis and electrochemical immunosensor was mainly discussed. Furthermore,the challenges and future development trends of BIA in practical application were elucidated. This review might provide new reference ideas and technical supports for the further development of BIA technique.
李兆周;袁云霞;韦玉花;张孝冲;陈秀金;王耀;牛华伟;李芳;高红丽;于慧春
河南科技大学食品与生物工程学院,河南省食品绿色加工与质量安全控制国际联合实验室,洛阳471000
分子印迹仿生亲和分析评述
Molecularly imprintedBiomimetic technologyAffinity analysisReview
《分析化学》 2024 (006)
763-777 / 15
洛阳市公益性行业科研专项项目(No.2202021A)、河南省优秀青年科学基金项目(No.202300410121)、国家自然科学基金项目(Nos.31701694,U1504330,31702218)、河南省青年人才托举工程项目(No.2020HYTP029)和河南省研究生教育改革与质量提升工程项目(No.HNYJS2020JD06)资助.Supported by the Special Fund for Scientific Research in the Public Interest from Luoyang City,Henan Province,China(No.2202021A),the Natural Science Foundation of Henan Province,China(No.202300410121),the National Natural Science Foundation of China(Nos.31701694,U1504330,31702218),the Youth Talent Support Project of Henan Province,China(No.2020HYTP029)and the Postgraduate Education Reform and Quality Improvement Project of Henan Province,China(No.HNYJS2020JD06).
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