分析化学2024，Vol.52Issue(6)：789-798,10.DOI:10.19756/j.issn.0253-3820.231227
数字化PCR芯片技术用于食源性细菌多重定量检测
Digital PCR Chip for Multiple Quantitative Detection of Foodborne Bacteria
摘要
Abstract
The presence of food-borne pathogenic bacteria poses a severe threat to human health. Therefore,it is essential to develop effective methods for bacteria detection. Herein,a method based on digital polymerase chain reaction (PCR) chip was developed for rapid and effective detection of three kinds of common food-borne bacteria (Staphylococcus aureus,Salmonella typhimurium and Listeria monocytogenes). Driven by centrifugal force,samples could be easily loaded and digitized in 15 min. PCR results showed that the positive microchambers in the chip could be identified after 25 thermal cycles. The chip had advantages in the quantitative detection of bacterial nucleic acid at low concentration,which could effectively avoid false negative results. Multiple detection of bacterial samples with concentration of 105-108 CFU/mL could also be realized using this chip. Compared with fluorescent quantitative PCR,this method had higher accuracy and sensitivity,especially for detection of targets at low concentration.关键词
数字化芯片/多重聚合酶链式反应/金黄色葡萄球菌/沙门氏菌/单增李斯特菌Key words
Digital chip/Multiple polymerase chain reaction/Staphylococcus aureus/Salmonella/Listeria monocytogenes引用本文复制引用
茅珍珍,胡文琪,唐曲,蒋汶君,秦玉岭,吴丽..数字化PCR芯片技术用于食源性细菌多重定量检测[J].分析化学,2024,52(6):789-798,10.基金项目
国家自然科学基金项目(No.31901056)和江苏省杰出青年基金项目(No.BK20220060)资助.Supported by the National Natural Science Foundation of China(No.31901056)and the Excellent Youth Foundation of Jiangsu Scientific Committee(No.BK20220060). （No.31901056）
