溴氰菊酯酶联免疫吸附分析和胶体金免疫层析分析方法的建立OA北大核心CSTPCD
Enzyme Linked Immunosorbent Assay and Gold Nanoparticle-based Lateral Flow Immunoassay for Rapid Detection of Deltamethrin
制备了9株分泌溴氰菊酯单克隆抗体的杂交瘤细胞株,选取灵敏度最高的单克隆抗体4D4E11建立了间接竞争酶联免疫吸附分析方法(ic-ELISA)和胶体金免疫层析分析方法(LFIA)用于检测溴氰菊酯.ic-ELISA的最优工作溶液为0.01 mol/L磷酸盐缓冲溶液(PB,NaH2PO4/Na2HPO4,pH 7.4),含20％甲醇和0.2 mol/L NaCl;LFIA的最优工作溶液为0.01 mol/L PB缓冲溶液(pH 7.4),含5‰Twee…查看全部>>
In this study,nine hybridoma cells secreting monoclonal antibodies against deltamethrin were prepared,and the monoclonal antibody 4D4E11 with best sensitivity was selected to develop indirect enzyme-linked immunosorbent assay (ic-ELISA) and gold nanoparticle-based lateral flow immunoassay (LFIA) for detection of deltamethrin. The optimal working buffer for ic-ELISA was 0.01 mol/L phosphate buffer (pH 7.4) containing 0.2 mol/L NaCl and 20％ methanol,whil…查看全部>>
崔盼盼;王贵绣;吴艳玲;张召贤;丁园;华修德
南京农业大学植物保护学院,南京210095南京农业大学植物保护学院,南京210095南京农业大学植物保护学院,南京210095安徽农业大学资源与环境学院,合肥230036南京农业大学植物保护学院,南京210095南京农业大学植物保护学院,南京210095
溴氰菊酯单克隆抗体间接竞争酶联免疫吸附法胶体金免疫层析法
DeltamethrinMonoclonal antibodyIndirect competitive enzyme-linked immunosorbent assayGold nanoparticle-based lateral flow immunoassay
《分析化学》 2024 (6)
828-837,中插20-中插23,14
国家自然科学基金项目(No.32102258)和安徽省重点研究与开发计划项目(No.2022i01020009)资助.Supported by the National Natural Science Foundation of China(No.32102258)and the Anhui Provincial Key Research and Development Program(No.2022i01020009).
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