分析化学2024，Vol.52Issue(7)：975-984,中插13-中插21,19.DOI:10.19756/j.issn.0253-3820.231071
基于β-二酮类铕(Ⅲ)配合物的单线态氧比率型荧光探针的合成及细胞成像应用
Design and Synthesis of a β-Diketonate-Europium(Ⅲ)Complex-based Ratiometric Fluorescent Probe for Singlet Oxygen and Its Application in Cell Imaging
刘威 1王华英 1车婉萌 1吴晶1
作者信息
- 1. 辽宁师范大学化学化工学院,大连 116029
- 折叠
摘要
Abstract
A ratiometric fluorescent probe([Eu(Hpfdap)3(CTMR-Phen-N)])was designed and synthesized for detection of singlet oxygen(1O2)with dimethyl anthracyl-containing β-diketone compound 1,1,1,2-pentafluoro-5-(9',10'-dimethyl-2'-anthracene)-3,5-pentanedione(Hpfdap)as recognition ligand and 5-amino-1,10-phenanthroline conjugated with Rhodamine as auxiliary ligand(CTMR-Phen-N).The results of spectral analysis showed that the europium(Ⅲ)complex had almost no fluorescence due to the presence of anthracene ring in the probe.After reaction with 1O2,the anthracene ring was oxidized to endoperoxide,and the fluorescence of the europium(Ⅲ)complex at 614 nm was restored,followed by the increase of quantum yield from 1.60%to 25.16%.The fluorescence intensity of Rhodamine at 575 nm remained unchanged before and after the reaction with 1O2.Therefore,the ratio of fluorescence intensity at 614 nm and 575 nm could be used to measure 1O2 by the developed rationmetric fluorescence method.The probe showed good selectivity for 1O2,and the detection limit(S/N=3)was as low as 3 μmol/L.The fluorescence imaging results demonstrated that the probe had low cytotoxicity and good membrane permeability,and could be applied to fluorescence imaging of 1O2 in Hela cells.关键词
铕(Ⅲ)配合物/比率荧光探针/单线态氧/荧光成像Key words
Europium(Ⅲ)complex/Ratiometric fluorescent probe/Singlet oxygen/Fluorescence imaging引用本文复制引用
刘威,王华英,车婉萌,吴晶..基于β-二酮类铕(Ⅲ)配合物的单线态氧比率型荧光探针的合成及细胞成像应用[J].分析化学,2024,52(7):975-984,中插13-中插21,19.
