分子影像学杂志2024，Vol.47Issue(6)：563-568,6.DOI:10.12122/j.issn.1674-4500.2024.06.01
牙槽骨骨髓间充质干细胞膜片复合PCL/HA支架具有良好早期成骨效果:基于动物模型研究
Alveolar bone marrow mesenchymal stem cells sheet combined with PCL/HA scaffold displays better osteogenesis effect in the early healing process:based on animal models
摘要
Abstract
Objective To investigate the repairing effect of alveolar bone marrow mesenchymal stem cells (Al-BMSCs) sheet combined with PCL/HA scaffold in rabbit mandibular defects. Methods Al-BMSCs sheets and Long bone marrow mesenchymal stem cells (Lon-BMSCs) sheets were cultured, PCL/HA scaffolds were produced by the fused deposition modeling method. Nine New Zealand rabbits were used to establish bilateral mandibular defect models. According to the implant materials, the rabbits were divided into 3 groups:Group A (PCL/HA scaffold), group B (Lon-BMSCs sheets combined with PCL/HA scaffold), group C (Al-BMSCs sheets combined with PCL/HA scaffold). A total of 18 samples were harvested, with 6 samples per group. All the experimental animals were sacrificed after 4 weeks. Gross observation, cone beam CT analysis, HE staining and Masson staining were used to evaluate the effect of bone regeneration. Results The results of cone beam CT showed that the value of BV/TV, Tb.Th, Tb.N in the group C were higher than that in group A and group B, and the difference was statistically significant (P<0.05). HE staining and Masson staining results showed that the osteogenesis in the group C was the most active, characterized by more newly formed bone and capillaries. Conclusion Al-BMSCs sheets combined with PCL/HA scaffold displays better osteogenesis effect in the early healing process, which is expected to become a new strategy for the regeneration of maxillofacial bone defects.关键词
牙槽骨骨髓间充质干细胞/细胞膜片/支架材料/骨缺损Key words
alveolar bone marrow mesenchymal stem cells/cell sheet/scaffold/bone defect引用本文复制引用
刘亚男,胡磊,霍文艳,王立凯,王海锋..牙槽骨骨髓间充质干细胞膜片复合PCL/HA支架具有良好早期成骨效果:基于动物模型研究[J].分子影像学杂志,2024,47(6):563-568,6.基金项目
中国康复研究中心青年基金项目(2021zx-Q12) （2021zx-Q12）
北京市医管局青年人才培养计划"青苗"计划(QML20191504) （QML20191504）
