广东医学2024，Vol.45Issue(6)：679-684,6.DOI:10.13820/j.cnki.gdyx.20240986
经后增殖方改善卵巢功能不全小鼠卵巢储备及凋亡状态的研究
Effect of Jinghouzengzhi Recipe on ovarian reserve and apoptosis in cyclophosphamide-induced ovarian insuffi-ciency mice
摘要
Abstract
Objective To investigate the effect of the Chinese herbal formula Jinghouzengzhi Recipe(JHZZR)on ovarian reserve and apoptotic status in cyclophosphamide-induced ovarian dysfunction mice.Methods Thirty female mice were randomly divided into cyclophosphamide(Cy)group,cyclophosphamide+JHZZR(Cy+JHZZR)group,and blank control group.Serum and ovarian tissues were collected after cervical dislocation.ELISA was used to measure serum levels of anti-Müllerian hormone(AMH),follicle-stimulating hormone(FSH),and estradiol(E2).The number of follicles at each stage in ovarian tissues was counted after HE staining.Real-time quantitative PCR was performed to de-tect the expression level of Caspase3 mRNA in ovarian tissues,and TUNEL assay was used to detect the apoptotic status of granulosa cells.Results Compared with the blank control group,the number of developing follicles in the ovaries of mice in the Cy group and Cy+JHZZR group significantly decreased,while the number of atretic follicles increased.The num-ber of primordial follicles and primary follicles in the Cy+J HZZR group increased compared to the Cy group,while the number of atretic follicles decreased,with statistical significance(P＜0.05).The AMH level in the serum of mice in the Cy+JHZZR group was higher than that in the Cy group[(6.12±1.66)ng/mL vs.(4.48±1.77)ng/mL,P＜0.05],while there was no significant difference in FSH and E2 levels.The relative expression levels of Caspase3 mRNA in the ovarian tissues of mice in the Cy group and Cy+JHZZR group were 5.86±1.33 and 4.45±1.02,respectively,both were higher than those in the blank control group(2.89±1.10,P＜0.01),with statistical significance between the two groups(P＜0.05).The apoptosis index(AI)of the blank control group,Cy group,and Cy+JHZZR group were(8.37±3.17)％,(16.85±5.46)％,and(12.80±3.34)％,respectively.The AI of the Cy group and Cy+JHZZR group were significantly higher than that of the control group,and the AI of the Cy+JHZZR group was lower than that of the Cy group,with statistical significance(P＜0.05).Conclusion JHZZR can improve ovarian reserve and reduce apoptotic status of ovarian cells in cyclophosphamide-induced ovarian dysfunction mice.关键词
经后增殖方/环磷酰胺/卵巢功能不全/卵巢储备/细胞凋亡Key words
Jinghouzengzhi Recipe/cyclophosphamide/ovarian insufficiency/ovarian reserve/apoptosis分类
医药卫生引用本文复制引用
李晖,欧莹,冼业星,黄岩,陈小燕,谢素嫣,郭新宇..经后增殖方改善卵巢功能不全小鼠卵巢储备及凋亡状态的研究[J].广东医学,2024,45(6):679-684,6.基金项目
广东省医学科研基金立项项目(A2023157) （A2023157）
