七氟烷后处理对脑缺血大鼠学习记忆功能和氧化应激反应的影响OACSTPCD

Objective To observe the effects of sevoflurane(Sevo)postconditioning on learning and memory function,neurological function,and oxidative stress response in rats with cerebral ischemia-reperfusion injury(CIRI).Methods Forty healthy male elderly SPF-grade SD rats were randomly divided into four groups:sham operation group(Sham group),cerebral ischemia-reperfusion model group(CIRI group),2％Sevo postconditioning group(2％Sevo group),and 3％Sevo postconditioning group(3％Sevo group),with 10 rats in each group.Except for the Sham group,the other three groups underwent left common carotid artery occlusion to establish the CIRI rat model.After model estab-lishment,the postconditioning groups inhaled 2％and 3％Sevo for 30 minutes,respectively,while the Sham and CIRI groups breathed spontaneously for 30 minutes.After 24 hours of reperfusion,neurological function was assessed using Gar-cia neurological function score;after 72 hours of reperfusion,learning and memory ability were tested using the Morris wa-ter maze experiment.Serum was collected,and the rats were sacrificed.Serum levels of lactate dehydrogenase(LDH),creatine kinase(CK),superoxide dismutase(SOD),and malondialdehyde(MDA)were measured by enzyme-linked immunosorbent assay(ELISA).Ischemic cortical tissue was collected to detect interleukin(IL)-1β,IL-6,and IL-10 levels by ELISA.Results After 24 hours of reperfusion,the Garcia score of the CIRI group was(9.932±0.349)points,which was significantly lower than that of the Sham group,and the Garcia score of the Sevo postconditioning groups was higher than that of the CIRI group,with the 2％Sevo group showing significant improvement(P＜0.05).After 72 hours of reperfusion,the escape latency of the CIRI group was significantly higher than that of the Sham group,and the number of platform crossings was significantly lower.The Sevo postconditioning groups showed a significant reduction in escape latency and an increase in platform crossings,with the 2％Sevo group showing significant improvement(P＜0.05).Serum levels of LDH,CK,MDA,and brain tissue IL-1β,IL-6,IL-10 in the CIRI group were significantly higher than those in the Sham group[(614.89±76.48)U/L,(1 369.52±218.03)U/L,(10.68±1.25)nmol/mL,(2.91±0.48)pg/mg,(2.89±0.69)pg/mg,(11.62±1.94)pg/mg],while serum SOD levels were significantly lower(89.15±6.12 U/mL),with statistically significant differences(P＜0.05).The Sevo postconditioning groups showed trends opposite to those of the CIRI group,with significant effects observed at the 2％Sevo concentration(P＜0.05).Conclusion Sevoflurane postconditioning exerts a significant neuroprotective effect on CIRI rats,reducing oxidative stress responses,improving learning and memory abilities,and alleviating neurological damage and brain inflammation.The op-timal effect was observed at a 2％Sevo concentration.The underlying mechanisms may be related to its anti-inflammatory and antioxidant properties.