广东医学2024，Vol.45Issue(6)：698-705,8.DOI:10.13820/j.cnki.gdyx.20240150
甲硫氨酸代谢轴关键酶在多发性骨髓瘤中的作用
The role of the key enzymes of methionine metabolism axis in multiple myeloma
摘要
Abstract
Objective To investigate whether methionine metabolic axis could be a potential therapeutic target for multiple myeloma(MM).Methods Ten MM patients(newly diagnosed and treatment-responsive groups)were se-lected from the Department of Hematology,and five healthy individuals who voluntarily donated bone marrow supernatant from the Affiliated Hospital of Guangdong Medical University were chosen as the control group.Targeted quantitative metabolomics analysis of bone marrow supernatants from the three groups was performed using ultra-high performance liq-uid chromatography-tandem mass spectrometry(UPLC-MS/MS).Multivariate statistical analysis was conducted on the metabolomics data.Human MM-related high-throughput gene chip data from the NCBI GEO database were analyzed using online tools Cell Ranger and Seurat software,and bioinformatics analysis techniques were used to identify common differential genes.Key genes related to the methionine metabolism axis in MM were screened.Results The levels of me-thionine and cysteine in the bone marrow supernatants of MM patients were significantly elevated(P＜0.05).Enzymes involved in the methionine cycle,including methionine adenosyltransferase 2A(MAT2A),adenosylhomocysteinase(AH-CY),and protein arginine methyltransferase 1(PRMT1),were found to be associated with MM progression.Conclusion The key enzymes MAT2A,AHCY,and PRMT1 may influence the biological behavior and disease progression of MM by regulating methionine metabolism.关键词
多发性骨髓瘤/甲硫氨酸循环/生物信息学/甲硫氨酸腺苷转移酶2A/腺苷同型半胱氨酸酶Key words
multiple myeloma/methionine cycle/bioinformatics/methionine adenosyltransferase 2A/adenosyl-homocysteinase分类
医药卫生引用本文复制引用
袁鑫,赵国鑫,岑玉蓉,郝嘉懿,李宁,冯锦山,黄金棋,何红华..甲硫氨酸代谢轴关键酶在多发性骨髓瘤中的作用[J].广东医学,2024,45(6):698-705,8.基金项目
国家自然科学基金资助项目(82270143) （82270143）
广东省基础与应用基础研究基金资助项目(2022A1515220122) （2022A1515220122）
湛江市科技计划项目(2022A01019) （2022A01019）
