高师理科学刊2024，Vol.44Issue(6)：68-73,6.DOI:10.3969/j.issn.1007-9831.2024.06.011
高山红景天不同部位愈伤培养及代谢差异
Callus culture and metabolism difference of different parts of Rhodiola sachalinensis A.Bor
摘要
Abstract
Leaf and stem segments of Rhodiola sachalinensis A.Bor were used as experimental materials to study callus induction and growth of Rhodiola sachalinensis under different mass concentrations of hormones,and non-targeted metabolomics analysis was performed based on GC-MS technology to comprehensively evaluate the differential metabolites of leaves and roots.The results showed that the optimal treatment conditions for leaf induction were MS+3.0 mg·L-1 NAA+7.0 mg·L-1 6-BA,and the healing rate reached 87.67%.The optimal treatment conditions for stem were MS+1.0 mg·L-1 NAA+5.0 mg·L-1 6-BA,and the healing rate reached 86.33%.The best hormone combinations for callus growth of leaves and stems were MS+0.5 mg·L-1 NAA+7.0 mg·L-1 6-BA.Stem segments showed stable and high levels during callus induction and callus growth.A total of 187 metabolites were isolated from the roots and leaves of Rhodiola sachalinensis,and 11 different metabolites were screened out.The metabolites with higher content in leaves are acids,while those with higher content in roots are sugars and alcohols.The experimental results are expected to provide theoretical experience for the development and utilization of local Rhodiola sachalinensis,and provide scientific guidance for further expanding the natural quantity and promoting the development of local Rhodiola sachalinensis industry.关键词
高山红景天/愈伤组织/差异代谢物/叶/茎Key words
Rhodiola sachalinensis A.Bor/callus/differential metabolites/leaves/stems分类
农业科技引用本文复制引用
辛宇琦,常秋阳,龚覃然基,孙瑛骏,马明佳,陈思凡,穆立蔷..高山红景天不同部位愈伤培养及代谢差异[J].高师理科学刊,2024,44(6):68-73,6.基金项目
黑龙江省级大学生创新训练项目(S202210225116) （S202210225116）
