广西科学院学报2024，Vol.40Issue(2)：135-144,10.DOI:10.13657/j.cnki.gxkxyxb.20240709.005
龙眼叶黄酮类有效组分配伍体外降糖作用的研究
Study on Hypoglycemic Effect of Flavonoids Active Component Compatibility from the Leaves of Dimocarpus longan Lour.in vitro
摘要
Abstract
Based on the hypoglycemic activity in vitro,the compatibility ratio of kaempferol,quercetin and quercitrin of flavonoids from the leaves of Dimocarpus longan Lour.was optimized.The insulin resistance model of HepG2 cells was established.The induction of insulin resistance model by different concentrations of insulin and different induction time was investigated.The dosage of kaempferol,quercetin and quercitrin was used as the investigation factor,and the glucose consumption rate of insulin-resistant HepG2 cells was used as the index.The star design-response surface method was used to optimize the compatibility ratio of the three.The results showed that the optimal condition for establishing the insulin resistance model of HepG2 cells was 1×10-7 mol/L insulin induction for 24 h.The optimal compatibility ratio of kaempferol,quercetin and quercitrin in vitro was kaempferol 105.0 µmol/L,quercetin 78.9 μmol/L and quercitrin 194.0 μmol/L.The fitting equation of compatibility ratio optimization test was Y=20.94+1.55 × A-0.44× B-2.05×C-0.66 AB+1.68 × AC-1.53 ×A2-3.2 × B2-2.68 × C2.The combined use of kaempferol,quercetin and quercitrin predicted a glucose consumption rate of 21.52％in HepG2 cells,and the experimental value was(21.05±0.52)％.The optimal compatibility ratio has the highest glucose consumption rate and better hypo-glycemic activity for HepG2 cells,which could provide scientific methods and experimental basis for the effec-tiveness of D.longan Lour.leaves in the treatment of diabetes.关键词
龙眼叶/组分配伍/山柰酚/槲皮素/槲皮苷Key words
the leaves of Dimocarpus longan Lour./component compatibility/kaempferol/quercetin/quer-citrin分类
医药卫生引用本文复制引用
梁晶春,胡珏,安施佳,郑飘雪,陆春莲,梁炎丽,陈明伟,梁洁..龙眼叶黄酮类有效组分配伍体外降糖作用的研究[J].广西科学院学报,2024,40(2):135-144,10.基金项目
国家自然科学基金项目(82160771),广西壮瑶药重点实验室项目(桂科基字[2014]32号),国家中医药管理局高水平中医药重点学科建设项目"少数民族药学(壮药学)"(zyyzdxk-2023165),广西中医药多学科交叉创新团队项目(GZKJ2309),广西高等学校千名中青年骨干教师培育计划项目(桂教人[2019]5号),壮瑶药协同创新中心项目(桂教科研[2013]20号),广西壮族自治区民族药资源与应用工程研究中心项目(桂发改高技函[2020]2605号),广西重点研发计划项目(桂科AB21196016),广西中医药重点学科壮药学项目(GZXK-Z-20-64),广西一流学科中药学(民族药学)项目(桂教科研[2018]12号),广西科技基地和人才专项(桂科AD20238058、桂科AD21238031)和广西中医药大学第三批"岐黄工程"高层次人才团队培育项目(202406)资助. （82160771）
