广西科学院学报2024，Vol.40Issue(2)：145-153,9.DOI:10.13657/j.cnki.gxkxyxb.20240709.006
超声辅助低共熔溶剂提取当归药渣粗多糖的工艺优化
Process Optimization of Deep Eutectic Solvent-based Ultrasound-assisted Extraction of Crude Polysaccharides from Angelica sinensis Residue
摘要
Abstract
In order to study the extraction process of crude polysaccharide from Angelica sinensis residue,the extraction rate of A.sinensis Residue Crude Polysaccharide(ARP)was used as an index to investigate the effects of the type of Deep Eutectic Solvent(DES),molar ratio of components,water content of DES,extrac-tion temperature,extraction time and liquid-to-material ratio on the extraction rate of ARP,and the extrac-tion process parameters were optimized by response surface analysis.The results showed that the deep eutec-tic solvent composed of choline chloride and ethylene glycol was more suitable for the extraction of ARP.The optimal extraction process was extraction temperature of 82 ℃,extraction time of 40 min,and molar ratio of 6:1.Under these conditions,the extraction rate of ARP was(14.43±0.10)％,and the total sugar content of ARP was(47.54±1.23)％.The extraction rate of ARP by traditional water extraction and alcohol precipita-tion method was(9.85±0.12)％,and the total sugar content of ARP was(25.45±1.37)％.Therefore,the deep eutectic solvent composed of choline chloride and ethylene glycol is a better extraction solvent,which can replace the traditional solvent for polysaccharide extraction and has practical application value.关键词
当归药渣/粗多糖/低共熔溶剂/超声辅助提取/响应面法Key words
Angelica sinensis residue/crude polysaccharides/deep eutectic solvent/ultrasound-assisted extrac-tion/response surface methodology分类
医药卫生引用本文复制引用
袁媛,凌丽燕,宾月景,孙翔,李司单,刘振华..超声辅助低共熔溶剂提取当归药渣粗多糖的工艺优化[J].广西科学院学报,2024,40(2):145-153,9.基金项目
广西科技计划项目-广西科技基地和人才专项(桂科AD2208001,AD22080019),广西青年科学基金项目(2023GXNSFBA026289),国家自然科学基金项目(31801313),中央引导地方科技发展资金项目(桂科ZY21195056),自治区级大学生创新训练项目(S202311354025),广西大学对口支援学科建设项目(2023W02)和梧州学院科研项目(2022A001)资助. （桂科AD2208001,AD22080019）
