广西科学院学报2024，Vol.40Issue(2)：181-188,8.DOI:10.13657/j.cnki.gxkxyxb.20240709.010
单酶法合成低分子量右旋糖酐的研究进展
Research Progress in the Synthesis of Low Molecular Weights Dextran by Single-Enzyme Method
摘要
Abstract
Low molecular weight dextran is synthesized by acid hydrolysis/enzymatic hydrolysis of glucose polymers catalyzed by Dextransucrase(DSR)using sucrose as a substrate.The molecular weight is 10 000-20 000 Da,which is widely used in medicine,food and other industries.In this article,the research progress on single enzyme synthesis of dextran were reviewed,including the advantages and disadvantages of microbial/enzymatic production of dextran,the efficient expression of DSR,the process regulation of single enzyme syn-thesis of dextran,the immobilization technology,the structure and catalytic mechanism of DSR,as well as the current research status of single enzyme synthesis of low molecular weight dextran.Based on this,the idea of DSR single enzyme one-step synthesis of low molecular weight glucan is proposed.With the help of artificial intelligence technology,more detailed DSR structure information is obtained.On the basis of further analy-zing the structure,function and catalytic mechanism of DSR,it is designed and improved reasonably,and the enzymatic properties are optimized to realize the direct synthesis of low molecular weight dextran by single enzyme one-step method,which provides new theoretical support for the production of low molecular weight dextran.关键词
单酶法/右旋糖酐蔗糖酶/低分子量右旋糖酐/一步法Key words
single-enzyme method/dextransucrase/low molecular weight dextran/one-step method分类
生物科学引用本文复制引用
秦艳,冼亮,郑元涛,吴晶桃,李亿,梁戈,王青艳..单酶法合成低分子量右旋糖酐的研究进展[J].广西科学院学报,2024,40(2):181-188,8.基金项目
国家自然科学基金项目(32360560),中央引导地方科技发展专项(桂科ZY23055011)和广西科学院基本业务经费项目(2021YBJ703,2021YFJ1202)资助. （32360560）
