内热针干预腰椎间盘突出症大鼠模型的效果及其潜在机制OACSTPCD
Effect of inner-heat acupuncture for the intervention of rats model with lumbar disc herniation and its potential mechanism
目的 探讨内热针干预腰椎间盘突出(LDH)大鼠模型的效果及其潜在机制.方法 将40只大鼠随机分为正常组、模型组、内热针组、电针组,每组10只.除正常组外,给予其余3组建立LDH模型.建模后,分别给予内热针组与电针组大鼠内热针与电针治疗,不给予正常组与模型组大鼠任何干预.干预2个疗程后,观察各组大鼠背根神经节组织病理变化,检测各组大鼠热刺激缩足阈值(TPWL)、神经传导速度、血清白细胞介素(IL)-6和IL-1β水平、背根神经节组织速激肽受体(NK)1和NK2蛋白表达水平.结果 模型组大鼠神经元细胞肿胀及细胞凋亡明显,细胞核不规则改变且核仁模糊,胞浆中尼氏小体排列混乱并呈空泡形态,内热针组及电针组大鼠的上述改变有所改善.与正常组大鼠比较,其余3组大鼠TPWL降低,感觉和运动神经传导速度均减慢,血清IL-6、IL-1β水平及背根神经节组织NK1、NK2蛋白表达水平升高.模型组、电针组及内热针组大鼠的TPWL依次升高,感觉和运动神经传导速度依次增快,血清IL-6、IL-1β水平及背根神经节组织NK1、NK2蛋白表达水平依次降低(P<0.05).结论 内热针可缓解LDH大鼠模型的疼痛,提高其感觉和运动神经传导速度,其作用机制可能与抑制NK1/NK2表达而减轻炎症反应有关.
Objective To investigate the effect of inner-heat acupuncture for the intervention of rats model with lumbar disc herniation(LDH)and its potential mechanism.Methods A total of 40 rats were randomly assigned to normal group,model group,inner-heat acupuncture group,or electric acupuncture group,with 10 rats in each group.Except for the normal group,LDH model was established in the remaining 3 groups.After modeling,rats in the inner-heat acupuncture and electric acupuncture groups received inner-heat acupuncture and electric acupuncture for treatment,respectively,whereas neither the normal group or the model group received any intervention.After 2 treatment courses of intervention,pathological changes of dorsal root ganglion tissues were observed in rats of various groups.Thermal paw-withdrawal latency(TPWL),nerve conduction velocity,serum interleukin(IL)-6 and IL-1β levels,tachykinin receptor(NK)1 and NK2 protein expressions in dorsal root ganglion tissues were detected in rats of various groups.Results Rats in the model group presented as swelling neurons and significant cell-apoptosis neurons,irregular-change and nucleolus-ambiguity in cell nucleus,disordered and cavity-form Nissl body in cytoplasm,whereas rats in the inner-heat acupuncture and electric acupuncture groups obtained ameliorated changes as above.Compared with the normal group,rats in the remaining groups obtained decreased TPWL,decreased sensory and motor nerve conduction velocity,elevated serum IL-6 and IL-1β levels,and elevated protein expressions of NK1 and NK2 in dorsal root ganglion tissues.The model group,the electric acupuncture group,and the inner-heat acupuncture group exhibited elevated TPWL successively,increased sensory and motor nerve conduction velocity,decreased serum IL-6 and IL-1β levels,and decreased protein expressions of NK1 and NK2 in dorsal root ganglion tissues(P<0.05).Conclusion Inner-heat acupuncture can relive LDH rats model's pain,improve their sensory and motor nerve conduction velocity.Its mechanism may be related to the inhibition of NK1/NK2 expression and thereby relieving inflammatory responses.
董飞;李娜;刘晓素;刘业双;邓玉龙
石家庄市中医院针灸推拿康复科,河北省石家庄市 050051河北省中医院骨科,河北省石家庄市 050051
基础医学
腰椎间盘突出内热针神经传导疼痛炎症反应速激肽受体1速激肽受体2大鼠
Lumbar disc herniationInner-heat acupunctureNerve conductionPainInflammatory responsesTachykinin receptor 1Tachykinin receptor 2Rats
《广西医学》 2024 (005)
721-726 / 6
石家庄市科学技术研究与发展计划项目(191461143)
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