河南农业科学2024，Vol.53Issue(7)：142-148,7.DOI:10.15933/j.cnki.1004-3268.2024.07.016
贵州光枝勾儿茶内生真菌Diaporthe sp.代谢产物分离鉴定及其抑菌活性研究
Isolation,Identification and Antibacterial Activity Research of Metabolites of Endophytic Fungus Diaporthe sp.from Berchemia polyphylla var.leioclada in Guizhou
摘要
Abstract
The aim is to investigate the chemical constituents of the metabolites of endophytic fungus Diaporthe sp.QX4G6 from Berchemia polyphylla var.leioclada in Guizhou,which has antibacterial activity,and to screen the antibacterial active compounds.After expanding culture of the strain Diaporthe sp.QX4G6,the metabolites were isolated and purified by various separation methods,and their structures were identified by spectrography combined with literature reports.The bacteriostatic activity of the monomers against E.coli and Salmonella was determined by microbroth dilution method and plate coating method,respectively.The results showed that four monomer compounds were isolated and purified from the metabolites of Diaporthe sp.QX4G6.The compounds were analyzed by NMR and MS.They were identified as dibutyl phthalate(compound 1),1-[(12E,16E)-12,16-eicosadienoyl]-2-[(E,E)-7,10-octadecadienoyl]-3-stearacylglycerol(compound 2),3-(4-toluoxy)-propionic acid(compound 3),tyrosol(compound 4).The results of bacteriostatic test showed that compounds 1-3 displayed certain inhibitory activity against E.coli and Salmonella,and the inhibitory effect on Salmonella was stronger under the same conditions.Compound 3 showed the strongest antibacterial activity with MIC(minimum inhibitory concentration)of 7.81µg/mL and 3.91µg/mL,and MBC(minimum bactericidal concentration)of 15.63 µg/mL and 7.81 µg/mL,respectively.In summary,all the four compounds were isolated from the metabolites of endophytic fungus Diaporthe sp.for the first time,and three compounds showed certain antibacterial activity on E.coli and Salmonella in vitro,which provided a theoretical basis for further development and utilization of endophytic fungal metabolites from Berchemia polyphylla var.leioclada.关键词
光枝勾儿茶/内生真菌/茎点霉属/抑菌活性/抑菌化合物Key words
Berchemia polyphylla var.leioclada/Endophytic fungus/Diaporthe sp./Antifungal activity/Antibacterial compound分类
生物科学引用本文复制引用
陶小艳,张涛,周思旋,唐远江,卢昱希,青成欣,史开志,赵宇,杨粤黔..贵州光枝勾儿茶内生真菌Diaporthe sp.代谢产物分离鉴定及其抑菌活性研究[J].河南农业科学,2024,53(7):142-148,7.基金项目
国家自然科学基金项目(31960716) （31960716）
贵州省农业科学院国家自然科学基金后补助资金专项项目(黔农科院国基后补助[2021]49号) （黔农科院国基后补助[2021]49号）
