基于网络药理学探讨诃子治疗慢性阻塞性肺疾病的作用机制OA

Objective:To study on the mechanism of Hezi(Chebulae Fructus)in treating chronic obstructive pulmonary disease(COPD)based on network pharmacology.Methods:The chemical ingredients of Hezi were retrieved through Traditional Chinese Medicine Sys-tems Pharmacology Database and Analysis Platform(TCMSP),and the target points of Hezi were obtained from the Swiss Target Predic-tion database.The targets of COPD were predicted by using OMIM,GeneCards,and DisGeNET databases.The intersection targets of He-zi and COPD were obtained by using the microbiome platform.A protein-protein interaction(PPI)network was constructed by importing the intersection targets into the STRING database.A topology analysis was performed based on the Centispace plugin to screen the core targets of Hezi for treating COPD.Gene Ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)analysis was per-formed by using the DAVID database.Results:There were 8 active ingredients and 333 targets of action in Hezi,including tannic acid,sennoside E,7-dehydrogustosterol,etc.There were 8,805 targets for COPD,and 253 targets for its intersection with Hezi.Ten core tar-gets were identified through screening,including signal transducer and activator of transcription 3(STAT3),serine/threonine kinase 1(AKT1),and mitogen activated protein kinase 3(MAPK3).GO functional analysis is mainly enriched in cell apoptosis,G-protein cou-pled serotonin receptor activity,RNA polymerase II transcription factor activity,etc.The KEGG pathway mainly included the PI3K Akt signaling pathway,HIF-1 signaling pathway,and ErbB signaling pathway.Conclusion:Hezi exerts therapeutic effects on COPD through multiple ingredients,pathways,and targets.