湖南农业大学学报（自然科学版）2024，Vol.50Issue(3)：35-40,6.DOI:10.13331/j.cnki.jhau.2024.03.005
DNA条形码技术在蛇类鉴别中的应用
Application of DNA barcoding in the identification of snake
摘要
Abstract
7 329 sequences of snake cytochrome b gene(Cytb)were downloaded from GenBank database of NCBI and compared and pruned according to MT765098.1 sequence.We obtained 4 665 snake Cytb sequences,and calculated the nucleotide saturation,genetic diversity,and intraspecific and interspecific genetic distance of Cytb sequences to construct phylogenetic trees.The results showed that,based on Kimura 2-Parameter model,the average intraspecific genetic distance of snakes was 3.3%and generally less than 6.7%.The average interspecific genetic distance is 19.96%,which is generally higher than 9.3%,indicating that there are great differences in genetic distance between species of snakes.According to the genetic distance between species,23 subspecies of snakes were identified.Pareas and Hydrophis contain composites,and Atractus dunni,A.iridescen and A.occidentali are sister species.关键词
蛇/细胞色素b基因/DNA条形码/遗传距离Key words
snake/cytochrome gene(Cytb)/DNA barcoding/genetic distance分类
生物科学引用本文复制引用
李晓冰,方来杉,陈洪博,吴琼,尹会方,林秀娇,李焰..DNA条形码技术在蛇类鉴别中的应用[J].湖南农业大学学报（自然科学版）,2024,50(3):35-40,6.基金项目
龙岩学院博士启动项目(LB2022009) （LB2022009）
