湖南农业大学学报（自然科学版）2024，Vol.50Issue(3)：41-46,6.DOI:10.13331/j.cnki.jhau.2024.03.006
外源GA3处理对辣椒半矮秆突变体表型和生理特征的影响
Effects of exogenous GA3 treatment on phenotype and physiological characteristics of a semi-dwarf mutant of pepper
摘要
Abstract
In this study,four concentrations(1.0,2.0,4.0,8.0 mg/L)of GA3 solution were used to treat the seeds of the semi-dwarf mutant E483,respectively,and the seedlings of E483 were sprayed with 2.0,4.0,6.0,8.0,10.0,12.0 mg/L GA3 solution,respectively,and wild-type 6421(CK1)and 0.0 mg/L GA3-treated E483(CK2)was taken as the control.The seed germination and plant phenotype of E483 were investigated under GA3 solution treatment,and the soluble sugar,soluble protein and MDA contents,as well as the activities of SOD,CAT,POD and GR were determined in the leaves of the seedlings.The results showed that the germination potential of E483 seeds was significantly lower than that of 6421 seeds,and the germination potential of E483 seeds treated with 2.0、4.0、8.0 mg/L GA3 solution could be restored to that of 6421 seeds.The height and leaf area of E483 plants were smaller than those of 6421,and the GA3 solution treatment could induce the elongation of the internodes of the stalks,stem thickening,and the increase of the leaf area of E483 but GA3 solution treatment could not change the number of nodes of E483.The plant height of E483 treated with 8.0 mg/L GA3 solution showed no significant difference from that of the 6421.The stem diameter of E483 treated with 2.0 mg/L GA3 solution also showed no significant difference from that of the 6421.Additionally,the leaf width and leaf area of E483 treated with 6.0 mg/L GA3 solution did not significantly differ from 6421.The contents of GA1,GA3,GA20,GA29 and GA34 in E483 seedlings were significantly lower than those in 6421,suggesting that the mutant was a GA synthesis defective mutant.The contents of soluble sugar and soluble protein,as well as the activities of SOD,CAT,POD and GR in E483 leaves,were significantly higher than those of 6421,while the MDA content was significantly lower than that of 6421.The soluble sugar,soluble protein contents and SOD,CAT,and POD activities in E483 leaves treated with 10.0 mg/L GA3 were all the highest.关键词
辣椒/半矮秆突变体/GA3/表型/生理指标Key words
pepper/semi-dwarf mutant/GA3/phenotype/physiological index分类
农业科技引用本文复制引用
廖铭宇,徐欢,张俊华,曹超政,程纬韬,周书栋,杨博智..外源GA3处理对辣椒半矮秆突变体表型和生理特征的影响[J].湖南农业大学学报（自然科学版）,2024,50(3):41-46,6.基金项目
湖南省自然科学基金项目(2022JJ30301) （2022JJ30301）
