湖南农业大学学报（自然科学版）2024，Vol.50Issue(3)：83-88,6.DOI:10.13331/j.cnki.jhau.2024.03.012
脂多糖处理时间对山羊瘤胃上皮细胞损伤的影响
Anti-damage effects of goat rumen epithelial cells induced by lipopolysaccharide
摘要
Abstract
Goat rumen epithelial cells(GRECs)were cultured in medium containing 1 μg/mL of LPS for 3 h,6 h and 9 h to detect cell viability,antioxidant indices,genes expression of inflammatory factors and tight junction protein.The results showed as follows:1)The viability of GRECs in the group treated with LPS for 3 h was significantly lower than that in groups treated with LPS for 6 h and 9 h,but there was no significant difference in GRECs activity between groups treated with LPS for 6 h and 9 h.2)The glutathione peroxidase(GSH-PX)activity of GRECs treated with LPS for 3 h was extremely significantly lower than that in cells treated with LPS for 6 h and 9 h(P<0.01),whereas the malondialdehyde(MDA)content,on the contrary,was extremely significantly higher in cells treated with LPS for 3 h.The superoxide dismutase(SOD)and catalase(CAT)activities of GRECs significantly increased and the reactive oxygen(ROS)concentration extremely decreased with the prolongation of LPS treatment time.3)The relative expression levels of TNF-ɑ and IL-1β in GRECs treated with LPS for 3 h were significantly higher than that in cells treated with LPS for 6 h and 9 h.The relative expression of IL-1β was significantly decreased with the extension of LPS treatment time.The relative expression levels of IL-6 had no significant difference among all group.4)The distribution of ZO-1 was low in GRECs treated with LPS for 3 h,but increased with the extension of LPS treatment time.The relative expression levels of Claudin-1 in GRECs treated with LPS for 3 h were significantly higher than that in cells treated with LPS for 6 h and 9 h.The results showed that the GRECs could alleviate oxidative damage by improving their own antioxidant and anti-inflammatory capacities with the extension of LPS treatment time,then improve barrier function of the cells.关键词
脂多糖/山羊瘤胃上皮细胞/抗氧化性能/抗炎性/紧密连接蛋白Key words
lipopolysaccharide/goat rumen epithelial cells/oxidation resistance/anti-inflammatory effect/tight junction protein分类
农业科技引用本文复制引用
占今舜,江浩筠,贾浩滨,王海波,谷志勇,潘月,钟小军,马月辉,霍俊宏..脂多糖处理时间对山羊瘤胃上皮细胞损伤的影响[J].湖南农业大学学报（自然科学版）,2024,50(3):83-88,6.基金项目
江西现代农业科研协同创新专项(JXXTCXQN202203) （JXXTCXQN202203）
江西省现代农业产业技术体系建设专项(JXARS-13-肉羊岗位) （JXARS-13-肉羊岗位）
