红外与毫米波学报2024，Vol.43Issue(3)：415-422,8.DOI:10.11972/j.issn.1001-9014.2024.03.016
可见-近红外二区双通道荧光成像系统
Visible-NIR-Ⅱ dual-channel fluorescence imaging system
摘要
Abstract
A dual-channel macroscopic imaging system has been developed based on optical methods,which can simultaneously capture the visible light and near-infrared second window(NIR-Ⅱ)fluores-cence.It could provide high-quality bright-field real-time images with the NIR-Ⅱ fluorescence informa-tion,addressing the significant disparity issue between fluorescence and bright-field images in conven-tional NIR-Ⅱ macroscopic imaging systems.In the experiment,the anti-scattering capability and imag-ing performance of NIR-Ⅱ fluorescence signals of indocyanine green(ICG)were tested using different thicknesses of adipose tissues in the band of 1 100-1 700 nm and 1 300-1 700 nm respectively.Subse-quently,the dual-channel macroscopic imaging system was used to obtain lymph node images of mouse and rat models,simulating the lymph node resection surgery and mimicking the process of ab-dominal lymph node clearance.Finally,different thicknesses of biological adipose tissues were added to the rat model to simulate the presence of adipose tissues covering the lymph nodes during actual sur-gery,and the penetration capability of the dual-channel system was observed.The visible and near-infrared second window dual-channel fluorescence imaging system provided the intuitive visual infor-mation to the operator,reducing surgery time and improving the patient prognosis,and held great po-tential for application in clinical surgical navigation.关键词
荧光成像技术/双通道宏观成像系统/近红外二区/临床手术导航Key words
fluorescence imaging/double-channel macroscopic imaging system/near-infrared second window/clinical surgical navigation分类
数理科学引用本文复制引用
刘雯,倪沪桅,钱骏..可见-近红外二区双通道荧光成像系统[J].红外与毫米波学报,2024,43(3):415-422,8.基金项目
国家自然科学基金(61975172和82001874),中央高校基本科研业务费专项资金(2020-KYY-511108-0007),浙江省自然科学基金(LQ21F050007) Supported by the National Natural Science Foundation of China(61975172 and 82001874),Fundamental Research Funds for the Central Universities(2020-KYY-511108-0007)and Zhejiang Provincial Natural Science Foundation of China(LQ21F050007) （61975172和82001874）
