杭州师范大学学报（自然科学版）2024，Vol.23Issue(4)：402-407,6.DOI:10.19926/j.cnki.issn.1674-232X.2023.10.131
肌酸相关基因在小鼠中枢神经系统中的表达模式分析
Analysis of the Expression Patterns of Creatine-related Genes in the Mouse Central Nervous System
摘要
Abstract
Creatine is able to maintain adenosine triphosphate(ATP)balance in cells and plays a key role in cellular energy metabolism.Abnormal synthesis and transport of creatine can lead to creatine deficiency syndrome(CDS),resulting in neurodevelopmental delay and neurodegenerative diseases.To date,the expression patterns of creatine-related genes in the central nervous system(CNS)remain poorly identified.By screening the transcriptome database,it was initially found that creatine related genes Slc6a8,Gatm and Gamt were predominantly expressed in oligodendrocyte(OL)lineage cells.To test this finding,the expression patterns of Slc6a8,Gatm and Gamt in mouse brain and spinal cord tissues at different developmental stages were examined through RNA in situ hybridization.Immunohistochemistry confirmed that Slc6a8,Gatm and Gamt were mainly expressed in differentiated oligodendrocytes.The results suggest that oligodendrocytes may be the major creatine synthesizing cells in the CNS.Oligodendrocytes play an important role in the maintenance of creatine homeostasis and energy metabolism in the whole central nervous system.关键词
肌酸/中枢神经系统/少突胶质细胞/Slc6a8/Gatm/GamtKey words
creatine/central nervous system/oligodendrocyte/Slc6a8/Gatm/Gamt分类
生物科学引用本文复制引用
谢冰花,尹乔,陈佳,赵新,戴忠敏..肌酸相关基因在小鼠中枢神经系统中的表达模式分析[J].杭州师范大学学报（自然科学版）,2024,23(4):402-407,6.基金项目
浙江省教育厅科研项目(Y202351679). （Y202351679）
