家畜生态学报2024，Vol.45Issue(7)：50-58,9.DOI:10.3969/j.issn.1673-1182.2024.07.008
基于RNA-seq鉴定金黄色葡萄球菌和大肠杆菌型乳房炎的关键应答基因
Identification of Key Response Genes for S.aureus and E.coli-type Mastitis in Cattle Using RNA-seq
摘要
Abstract
To explore key response genes related to bovine mastitis induced by Staphylococcus aureus(S.aureus)and Escherichia coli(E.coli)by RNA sequencing technology,and lay a foundation for the discovery of biomarkers related to the prevention and treatment of bovine mastitis,in this study,the MAC-T cells were treated with S.aureus in vitro,and RNA sequencing was performed.At the same time,9 RNA sequencing data(download from NCBI SRA)of bovine mammary epithelial cells treated with S.aureus and E.coli were collected from the public database,and the key response genes of S.aureus and E.coli mastitis were discovered by using bioinformation analysis techniques.The results showed that 449 differentially expressed genes(DEGs)were detected in mammary epithelial cells treated with S.aureus,among which 223 were up-regulated and 226 were down-regulated.Another 347 DEGs were detected in cells treated with E.coli,among which 116 were up-regulated and 231 were down-regulated.Functional enrichment analysis showed that these DEGs were mainly enriched in IL-17 signaling pathway.Protein in-teraction analysis(PPI)revealed that the 383 exclusive DEGs related with mastitis induced by S.aureus formed 21 regulatory networks,among which the largest regulatory network contained 67 DEGs.In addi-tion,281 exclusive DEGs related with mastitis induced by E.coli formed 16 regulatory networks,among which the largest regulatory network contained 33 DEGs.Cytoscap and CytoHubba were used to further screen out 10 key response genes related with mastitis induced by S.aureus or E.coli.The discovery of these key response genes will lay a foundation for further elucidating the mechanism of occurrence and de-velopment of S.aureus-and E.coli-mastitis.关键词
奶牛乳房炎/金黄色葡萄球菌/大肠杆菌/牛乳腺上皮细胞Key words
bovine mastitis/Staphylococcus aureus/Escherichia coli/bovine mammary epithelial cell分类
农业科技引用本文复制引用
师志海,王文佳,刘洁,王亚州..基于RNA-seq鉴定金黄色葡萄球菌和大肠杆菌型乳房炎的关键应答基因[J].家畜生态学报,2024,45(7):50-58,9.基金项目
河南省农业科学院自主创新项目(2022ZC47) （2022ZC47）
