家畜生态学报2024，Vol.45Issue(8)：8-15,8.DOI:10.3969/j.issn.1673-1182.2024.08.002
利用mtDNA COI、Cytb和D-loop条形码探讨地方牛的遗传多样性和系统发育关系
Exploring Genetic Diversity and Phylogenic Relationships of Indigenous Cattle using mtDNA COI,Cytb and D-loop Barcodes
摘要
Abstract
This study aimed to investigate the genetic diversity of local cattle breeds.In this experi-ment,blood and hair samples were collected in different regions from Wannan cattle,Leiqiong cattle,Naqu yak and Haizi buffalo,and genomic DNA was obtained.mtDNA COI,Cytb and D-loop genes were amplified by conventional PCR technique,and barcode sequences were obtained by Sanger sequencing,and then their genetic information was explored.The results showed that the A/T content of the mitochondrial genes of the four cattle species were significantly higher than the G/C content,with a significant anti-G bi-as,and all of them showed high genetic diversity,among which the Naqu yak was particularly outstand-ing.The use of COI and Cytb protein codons also showed a certain preference.The NJ phylogenetic tree constructed based on Cytb gene showed the best performance and was able to cluster the four cattle species better,which was more suitable for the exploration of phylogenetic relationships in this paper.关键词
地方牛/DNA条形码/遗传多样性/系统发育Key words
indigenous cattle breed/DNA barcode/genetic diversity/phylogeny分类
农业科技引用本文复制引用
张晓婷,彭珍珍,郭玛丽,袁朝海,蔡亚非,张威..利用mtDNA COI、Cytb和D-loop条形码探讨地方牛的遗传多样性和系统发育关系[J].家畜生态学报,2024,45(8):8-15,8.基金项目
国家重点研发计划(2018YFC1200201) （2018YFC1200201）
江苏省自然科学基金(BK20160722) （BK20160722）
国家自然科学基金(31701229) （31701229）
中央高校基本业务费(KYZ201723、KJQN201829) （KYZ201723、KJQN201829）
